摘要
目的建立测定康艾扶正颗粒中黄芪甲苷含量的HPLC-ELSD法。方法采用HPLC-ELSD法，pilenomene×Luna 5u C18色谱柱（150mm×4．60mm，5μm），以乙腈-水（34：66）为流动相，流速：1．0ml·min^-1。ELSD参数：漂移管温度110℃，气体流速2．8L·min^-1。结果在选择的色谱条件下黄芪甲苷与其它组分分离良好，对照品在2．064～10．32μg范围内呈良好的线性关系（r=0．9999），平均回收率为100．48％，RSD为1．06％。结论HPLC-ELSD法可用于康艾扶正颗粒中黄芪甲苷含量测定。
Aim A HPLC-ELSD method was developed for the quantitative determination of astragaloside Ivin Kangai Fuzheng Granules. Methods Astragaloside Ⅳ concentration was detected by using high performance evaporative light scattering detection The chromatographic conditions adopted were：phenomenne × Luna-C18 column（ 150 mm ×4.60 mm, 5 μm） ,a mobile phase of acetonitrile-water（34： 66） ,room temperature of the drift tube was set at 110℃ ,and the flow rate of nebulizing gas at 2.8 L · min^-1. Results The alibration curves for the quantitative determination of astragaloside IV was linear over the range of 2.064 - 10. 32 μg （r = 0. 9999）. The recovery was 100. 48% with RSD 1.06 %. Conclusion The developed method can be used for the quantitative determination of stragaloside Ⅳ in kanghi fuzheng granules.
出处
《安徽医药》
CAS
2006年第7期503-504,共2页
Anhui Medical and Pharmaceutical Journal
