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碱性β-甘露聚糖酶基因异源表达的研究 被引量：1

Heterogenous Expression of an alkaline β-mannanase Gene
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摘要 亚克隆的碱性β-甘露聚糖酶基因(man)来源于嗜碱芽孢杆菌N16-5,构建了大肠杆菌-枯草芽孢杆菌诱导型表达质粒pDG-man,在大肠杆菌JM109中获得了活性表达,经0.5 mmol/L的IPTG诱导后,可表达5 U/mL碱性β-甘露聚糖酶。重组大肠杆菌DE3-RIL(pDG-man)表达β-甘露聚糖酶水平是大肠杆菌JM109(pDG-man)的2倍。重组枯草芽孢杆菌WB600(pDG-man)可表达19.2 U/mL碱性β-甘露聚糖酶。 An alkaline β-mannanase gene from alkaliphilic Bacillus sp. N16-5 was subcloned into plasmid pDG148-Stu yielding hybrid pDG-man, An Escherichia coli JM109 harboring pDG-man was functionally expressed the alkaline β-mannanase with the enzyme activity of 5 U/mL after induced with 0.5 mmol/L IPTG. Subsequently, E. coli DE3-RIL and B. subtilis WB600 were used as host cells. E. coli DE3-RIL harboring pDG-man expressed a double enzyme of that E. coli JM109 did. B. subtilis WB600 harboring pDG-man expressed 19.2 U/mL of the enzyme.
作者 路志群 马延和 王正祥
机构地区 工业生物技术教育部重点实验室和江南大学生物工程学院
出处 《微生物学杂志》 CAS CSCD 2006年第3期9-11,共3页 Journal of Microbiology
基金 新世纪优秀人才支持计划(NCET-04-9704)
关键词 碱性甘露聚糖酶 基因表达 枯草芽孢杆菌 大肠杆菌 Alkaline β-mannanase Gene expression Bacillus subtilis Escherichia coli
分类号 Q939.124 [生物学—微生物学]
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参考文献9

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微生物学杂志
2006年 第3期

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