摘要
目的：研究p16及nm23在喉癌组织中的表达及其临床意义。方法：应用免疫组化S—P法检测抑癌基因p16及nm23在53佣喉癌组织中的表达，并与喉正常黏膜组织对比，结合喉癌的临床病理特征进行分析。结果：在喉癌中p16蛋白表达的阳性率为47．2％，nm23的阳性率为52．8％；中、低分化的鳞癌（Ⅱ～Ⅲ级）组p16阳性率低于高分化鳞癌（Ⅰ级）组（P〈0．01），有淋巴结转移组p16的表达阳性率低于无淋巴结转移组（P〈0．05）；TNM分期Ⅰ～Ⅳ期肿瘤的nm23表达率高于Ⅰ期肿瘤（P〈O．01）；颈淋巴结转移组nm23表达率低于非转移组（P〈0．01）。结论：p16蛋白表达与喉癌的细胞分化程度、颈淋巴结转移有关，与临床分期、临床分型无关；nm23的表达与临床分期及颈淋巴结转移有关，与临床分型、细胞分化程度无关。两者的检测对估计喉癌的恶性度、判断预后及指导治疗有一定意义。
Objective：To study the expression of p16 and nm23 and the clinical significance in laryngeal carcinoma. Methods： p16 and nm23 protein expressions in 53 cases of laryngeal carcinoma were detected by immunohistochemistry （SP method）,with normal laryngeal epithelium as control. Results：In laryngeal carcinoma, the positive rates of p16 and nm23 were 47.2% （25/53） and 52.8% （28/53）, respectively. The p16 positive rate in grade Ⅱ and Ⅲ carcinoma was lower than that in grade Ⅰ carcinoma （P〈0. 01）, in neck lymph nodes with metastasis was lower than in neck lymph nodes without metastasis （P〈（0.05）. The nm23 positive rate in PTNM Ⅰ and Ⅱ carcinoma was higher than that in PTNM Ⅲ and Ⅳ carcinoma （P〈0.01）, in neck lymph nodes with metastasis was lower than in neck lymph nodes without metastasis （P〈0.01）. Conclusion;The expression of p16 protein might be related to the degree of differentiation and cervical lymph node metastasis, the expression of nm23 might be related to the clinical staging and cervical lymph node metastasis. Detection of the above parameters could have significance in predicting the biological aggressiveness and clinical outcome of laryngeal carcinoma, which may guide to make the therapeutic decision.
出处
《广东医学院学报》
2006年第3期240-241,共2页
Journal of Guangdong Medical College
