摘要
目的:建立测定诺坤复片中雌二醇含量的胶束电动毛细管色谱法(MECC)。方法:采用胶束电动毛细管色谱法,测定条件为:电泳缓冲液为20mmol·L^(-1)硼砂缓冲溶液(pH=9.2),内含40mmol·L^(-1)十二烷基硫酸钠(SDS)及5%甲醇,分离电压为18kV,温度为25℃,空心熔融未涂层石英毛细管柱75μm(id)×35cm(有效长度),检测波长为280nm,进样方式为压力进样,进样时间5.0s,外标法定量。结果:雌二醇的线性范围为25～400μg·mL^(-1)(r=0.9998),平均回收率为100.1%,RSD为1.63%(n=3)。结论:该方法简便、准确、灵敏、适用于雌二醇的质量控制。
Objective：A useful method for determination of estradiol in estrofem tablets by micellar electrokinetic capillary chromatography（MECC） has been developed. Methods：MECC ,the buffer solution was 20 mmol ·L^-1 borate buffer （pH =9. 2） ,containing 40 mmol ·L^-1 sodium dodecyl sulfate （SDS） and 5% methanol. The effective length of 75 μm （id） fused silica capillary was 35 cm and the detective wavelength was 280 nm. The voltage and temperature was 18 kV and 25 ℃ respectively. Sample were injected into the capillary by applying pressure for 5.0 s. The concentrations were quantified by external standard method. Results： The calibration curve of estradiol was linear over the range of 25 -400μg · mL^-1 （r =0. 9998）. The average recovery was 100. 1% （n =3）. Conclusion：The method is simple, accurate and sensible, and can be used for quality control of the estradiol.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2006年第6期781-783,共3页
Chinese Journal of Pharmaceutical Analysis
