摘要
目的：建立一种快速、灵敏及检测范围广的高效液相色谱法测定人血浆中伏立康唑浓度的方法。方法：采用LC-10A（岛津）高效液相色谱仪，以尼群地平为内标，以Hypersil ODS柱（250mm×4．6mm，5μm）为分析柱；流动相为20mmol·L^-1磷酸二氢钾缓冲液（pH6．0）-乙腈（55：45）；流速为1．0mL·min^-1；进样量为50μL；检测波长为254nm。血浆样品用乙腈沉淀蛋白，离心后取上清液进样分析。结果：色谱峰分离良好，无内源性杂质干扰。线性方程为：Y：0．3124X-0．0693，r=0．9991（0．2～20．0mg·L^-1）；最低检测浓度为0．05mg·L^-1。平均方法回收率为94．6%～105．4％（n=15），日内、日间RSD≤9．2％。结论：本方法准确灵敏，操作简便、快速，适用于伏立康唑血药浓度的监测，也可用药动学研究。
OBJECTIVE To develop a HPLC method to determine the concentration of voriconazole in human plasma. METHODS A reversed phase C18 column （250 mm×4. 6 mm, 5μm） was used for the determination of voriconazole and nitrendipine （internal standard） with a mobile phase composed of 20 mmol·L^-1 phosphate buffer solution （pH 6. 0） - acetonitrile （55：45, v/v） at a flow rate of 1.0 mL·min^-1. The detector was operated at the 254 nm. Plasma samples were deposited down the protein with acetonitrile. RESULTS The chromatography was good and not interfered by the components of the plasma. Calibration curve was linear from 0. 2 to 20 mg L^-1. The recovery from rat plasma was 94. 6% -105.4% （n = 15）. The limit of detection was 0. 05 mg·L^-1. The intra- and inter-day precision （C. V. ） was less than 9. 2%. CONCLUSION The method is accurate, sensitive and convenient, and is suitable for the determination of voriconazole concentration in human plasma and pharmacokinetic study.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第7期836-839,共4页
Chinese Journal of Hospital Pharmacy
