摘要
AIM： To investigate whether Thyl recognizes oval cells in the fetal liver and to characterize the cultured Thy1 selected cells from E14 rat livers. METHODS： Thyl populations were analyzed by fluorescence activated cell sorter analysis. Thyl positive cells were isolated using magnetic beads. Hepatic markers were detected by Western blotting, immunocytochemistry and RT-PCR. RESULTS： The percentage of Thyl-positive cells decreased during early development of fetal rat liver （E13-E16）. E14 fetal livers contained 7.8% Thy1 positive cells, of which 61% were positive for α-fetoprotein （AFP） and 25% expressed albumin. The Thy1＋ population expressed oval cell markers c-Kit and CXCR4, liver enriched-transcription factors HNF1α and HNF6, hepatocytic markers albumin, AFP and cytokeratin 18, and biliary marker cytokeratin 19. Thy1- selected cells formed only mesenchymal colonies when plated on collagen and in serum-containing media. Thyl selected cells were able to form hepatic colonies positive for HNF1α, HNF6, albumin, AFP, cytokeratin 18, cytokeratin 19 and glycogen, when grown on STO feeder layers in serum free-media. CONCLUSION： Oval cells positive for Thyl are present in early liver embryonic stages.
瞄准：为了调查 Thy1 是否在胎儿的肝认出卵形的房间并且描绘，有教养的 Thy1- 从 E14 老鼠肝选择了房间。方法：Thy1 人口被荧光分析激活的房间 sorter 分析。Thy1 积极房间用磁性的祷告被孤立。肝的标记被西方的弄污，免疫细胞化学和 RT-PCR 检测。结果：Thy1 积极的房间的百分比在胎儿的老鼠肝(E13-E16 ) 的早开发期间减少了。E14 胎儿的肝包含了 7.8% Thy1 积极房间， 61% 为 alpha-fetoprotein (法新社) 和 25% 表示白朊是积极的。Thy1+ 人口表示了卵形的房间标记 c 工具包和 CXCR4，肝充实抄写的因素 HNF1alpha 和 HNF6， hepatocytic 标记白朊，法新社和 cytokeratin 18，并且胆汁的标记 cytokeratin 19。Thy1- 选择了房间形成的仅仅间充质的殖民地什么时候骨胶原上并且在包含浆液的媒介的 plated。选择房间能形成为 HNF1alpha 积极的肝的殖民地的 Thy1， HNF6，白朊，法新社， cytokeratin 18， cytokeratin 19 并且肝糖，当在浆液在 STO 喂食器层上成长时免费媒介。结论：为 Thy1 积极的卵形的房间在早肝是在场的胚胎的阶段。
基金
Supported by the Genesis Consortium for Cell Therapy, Israel Ministry of Science
