摘要
以新鲜小牛松质骨(FX)、脱抗原处理的牛松质骨载体(BC)及不同复合比例的重组合异种骨(RBX)植入Balb/c小鼠股部肌袋内,正常小鼠模拟手术做为对照组。术后7、14及28天,取材做组织学检查,同时以间接免疫荧光法测定外周血及脾脏淋巴细胞中Thy1、L3T4、Lyt2及Tac阳性细胞百分数。结果示:脱抗原载体植入组与正常对照组无明显差异。FX组阳性淋巴细胞百分数均明显升高,以Tac升高显著;组织学示局部免疫排斥而无骨诱导生成。RBX植入组Tac无增高,组织学见活跃的异位诱导成骨而无免疫排斥反应,但Thy1及Lyt2细胞荧光染色阳性率增高,以Lyt2增高明显,Th/Ts比值下降或倒置,为RBX中所含BMP所致。这一现象可能与机体对异种BMP及RBX不引起明显免疫排斥有关。Tac细胞阳性表达率对评价异种骨移植时免疫排斥反应有一定的意义。
Freshcalfcancelousbonexenograft(FX),antigenfreecancelousbonecarrier(BC)andreconstitutedbonexenograft(RBX)wereinplantedinthethighmusclepouchesofBalb/cmice.Histo-logicalexaminationsweredoneat7,14and28dayspostoperation,andindirectimmunofluorescencewasusedtodeterminethepositivecountsofThy1,L3T4,Lyt2,andTacTlymphocyteofthebloodandspleenatthesametime,withthenormalmouselymphocytesascontrol.Therewasnosignificantdifer-encebetweentheBCimplantedgroupandthenormal(control)group,whiletheFXgrouphadasignifi-cantincreaseratesofThy1、L3T4andLyt2,especialyofTaclymphocytes.TheRBXimplantedgrouphadnoincreaseofTacbutincreaseofThy1andLyt2.Th-Tsratiodecreased,anditwasprobablycausedbybovinebonemorphogeneticprotein(bBMP)intheRBX.Histologicaly,intenseimmunerejectionwasnotedinFXimplantedgroupbutnotintheothertwogroups.HeterotopicossificationwasnotedintheRBXimplantedgroup.FXincreasedintenseimmunologicrejectioninthehost,batRBXdidnot,thatmightbeduetothedecreaseofTh/TscausedbybBMP.Taclymphocytecountmayindicatetheim-munologicrejectionofbonexenografttransplantation.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1996年第8期453-456,共4页
Chinese Journal of Surgery
关键词
骨移植
移植免疫学
T淋巴细胞
BonetransplantationTransplantationimmunologyT-LymphocytesT-Lymphocytessubsets