摘要
以蛋白质得率为目标,综合考虑绿色木霉(TrichodermavirideNUA-051)与产朊假丝酵母(Candidautilis)2.281的共生特性及营养竞争,通过木霉发酵产物还原糖对菌体生长抑制的实验研究及发酵条件实验,确立了以酸解玉米秸秆为原料生产单细胞蛋白的发酵工艺:酸解玉米秸秆150g/L,木霉发酵40h接种酵母2%,(NH4)2SO425.0g/L,KH2PO46g/L、MgSO4·7H2O0.4g/L,通风量为4.5L/L·min为,搅拌转速为600r/min,pH5~6,温度35℃。木霉发酵时间40h,酵母发酵时间24h,发酵总周期64h。
Aiming at protein yield rate, the fermentation technology for single cell protein production from acidolysis maize straws was established through the experiments of product-restraining from reducing sugar, and fermentation conditions, and the symbiosis and nutrition competition between Trichodermaviridec NUA-051 and Candida utilis were taken into account. After Trichodermaviride growth 40h, 2% Candida utilis culture was inoculation, aacidolysis maize straws concentration is 150g/L, (NH4)2SO4 25.0g/L, KH2PO4 6g/L, MgSO4.7H2O 0.4g/L, aeration rate 4.5L/L·min, mixing speed, 600r/min, pH5-6, and temperature 35%. Candida utilis fermentaion time was 24h, and the total fermentation time was 64h.
出处
《食品科技》
CAS
北大核心
2006年第7期261-264,共4页
Food Science and Technology
基金
宁夏自然基金项目(NZ0509)