摘要
目的探讨流行性脑脊髓膜炎(简称流脑)的病原学快速检测鉴定及其分型的实验方法。方法24例流脑病例为实验组,63例其他脑膜炎病例为对照组,抽取患者脑脊液(CSF)进行常规及生化检测同时,直接涂片革兰染色镜检细菌,单克隆抗体标志乳胶凝集试验快速检测脑膜炎奈瑟菌polyosid ic抗原;细菌培养及API-NH系列鉴定脑膜炎奈瑟菌,并作单克隆抗体分型。结果实验组24份CSF中,乳胶凝集法阳性22份,对照组63份CSF,阴性61份,阳性2份,乳胶凝集法检测脑膜炎奈瑟菌poly-osid ic抗原诊断流脑,敏感性91.7%,特异性96.8%,阳性预测值0.92,阴性预测值0.97。鉴定分型均为A群。结论A群脑膜炎奈瑟菌是引起该地区流脑的主要菌种,单克隆抗体标志乳胶凝集法快速检测脑膜炎奈瑟菌polyosid ic抗原,具有较高敏感性和特异性。
Objective To investigate a rapid method for detecting and identifying pathogen of epidemic cerebrospinal meningitis and it＇ s grouping. Methods Twenty-four epidemic cerebrospinal meningitis patients （ experimental group）and sixty-three other typing meningitidis patients （control group）were examinated about the appearance,white blood cell, glucosum and protein with cerebrospinal fluid（CSF）. The bacterials are examined by Gram-stain. The polyosidic antigen of Neisseria meningitides were examined by latex agglutination assay. The Neisseria meningitidis were identlficated by bacterial culture and API-NH system, and were grouped by monoclonal antibody. Results Twenty-two copies CSF were positive in latex agglutination assay in experimental group, two copies were positive in control group. The sensitivity was 91.7%, the specificity was 96.8% , positive expectation was 0.92 and negative expectation was 0.97 in this assay. All of the Neisseria meningitidis were A group. Conclusion A group of Neisseria meningitidis is the main phathogenic bacterial of epidemic cerebrospinal meningitis in this area; Rapid latex agglutination assay marked with monoclonal antibody has higher sensitivity and specificity in examinating Polyosidic antigen of Neisseria meningitidis.
出处
《现代实用医学》
2006年第7期461-463,共3页
Modern Practical Medicine
关键词
脑脊髓炎
奈瑟球菌
脑膜炎
polyosidic抗原
抗体
单克隆
Epidemic cerebrospinal meningitis
Neisseria meningitidis
Polyosidic antigen
Monoclonal antibody
Agglutination assay
