摘要
目的探讨人发中锌的β-环糊精交联聚合物-树脂相分光光度测定法。方法以β-环糊精和环氧氯丙烷为原料合成了环糊精聚合树脂,将其作为显色剂2-眼5-溴-(2-吡啶偶氮)演-5-二乙氨基苯酚的载体,采用β-环糊精-2-眼5-溴-(2-吡啶偶氮)演-5-二乙氨基苯酚树脂相分光光度法测定人发中微量锌。结果树脂包结物的最大吸收峰位于570nm,表观摩尔吸光系数为8.63×105L/(mol·cm)。线性范围为4～720μg/L,线性回归方程为y=0.0011+0.1320x,相关系数为r=0.9984,RSD为1.0%～2.2%,加标回收率为99.5%～100.4%。结论该方法可用于人发中痕量锌的测定,操作简单,灵敏度高,准确性好,干扰少,结果令人满意。
Objective To develop a method for determination of trace zinc in human hair. Methods β-cyclodextrin polymer （β-CDP） was synthesized using epichlorohydrin as the cross-link agent and it was used as the carrier of 2-[5-bromo- （2-pyridylazo）]- 5-diethylaminophenol. Resin-phase spectrophotometric method with （β-CDP）and 5-Br-PADAP was used for determination of trace zinc. Results The maximum absorbance of inclusion resin was at 570 nm with apparent molar absorption of 8.63×105 L/（mol. era）. The Beer＇s law was obeyed in the range of 4-720 μg/L. The regression equation of calibration was y=0.001 1＋0.132 Ox, with a correlation coefficient of 0.998 4 and the recovery rates were 99.5%-100.4%. Conclusion The method is simple, sensitive, accurate and can be used for the determination of trace zinc in human hair with satisfactory results.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第4期363-364,共2页
Journal of Environment and Health
