摘要
目的 建立大鼠血浆中淫羊藿苷的检测方法。方法 采用RP-HPLC法测定大鼠血浆中的药物浓度，色谱柱为Lichrospher C18（4．6ram×250mm，5μm），流动相为甲醇-水-冰醋酸（45：55：0．5v／v），检测波长为270nm，流速为1ml·min^-1。结果 在1～96mg·L^-1范围内淫羊藿苷峰面积与浓度线性关系良好（r=0．9993），最低检测浓度为0．03mg·L^-1，绝对回收率为85．3％～96．6％，准确度为94．3％～101．2％，日内、日间RSD均小于10％。结论 本测定方法灵敏、准确、简便，适合于淫羊藿苷的药代动力学研究。
Objective To develop a simple, sensitive and rapid method for the determination of Icariin in rat plasma. Methods The plasma concentration of Icariin was determined by RP - HPLC method, the analysis involved a 250 mm × 4.6mm column packed with C18 （ 5 μm ） ,the mobile phase consisted of methyl alcohol -water- glacialacetic acid （45：55：0.5 ） ,the detection was performed at UV 270nm. Results A good linearity was obtained in the range of 1 ～ 96 mg/L （ r = 0. 9993 ） , the recovery was between 85.3 ～ 96.6% , the accuracy was between 94.3% ～ 101.2%. The relative standard deviations of within - day and between day were less than 10%, the minimal detectable concentration in rat plasma was 0.03 mg/L. Conclusion The result demonstrated that the method was sensitive, accurate and simple for the determination of Icariin in rat plasma, it was suitable for the pharmacokinetics study of Icariin.
出处
《青海医学院学报》
CAS
2006年第2期110-112,共3页
Journal of Qinghai Medical College
