摘要
为建立一种快速、灵敏、稳定性好的分光光度法测定血清镁，在表而活性剂TritonX-100存在下，用国产试剂偶氮胂Ⅰ作显色剂，EGTA掩蔽钙，在三乙醇胺．盐酸缓冲体系中以分光光度法测定血清镁。结果表明，该法显色络合物最大吸收波长为580nm，线性范围达3．29mmol／L，表观摩尔吸光系数为1.71 × 10^4 L·mol^-1· cm^-1。回收率为97．8％～102．4％，批内变异系数（CV）和批问变异系数分别为2．9％与3．4％，与MTB法（x）比较具有良好的相关性，y=1．03x-0．036，r=0．9947，P〉0．05，51例健康人血清镁含鞋（x±2s）为0．73～1．27mmol／L。应用该法进行血清镁测定具有灵敏度高、准确性好、试剂空白值低、操作快速简便等优点，适于血清镁的常规手工分析和自动分析。
To establish a simple, sensitive and stable method of determination of magnesium in serum by UVspectrophotometry, Arsenazo Ⅰ was used as a coloring reagent to determine magnesium by spectrophotometry in the presence of surfactant Triton X - 100. The results showed that the maximum absorption of the complex compound was at 580 nm, the linearity was 0 - 3.29 mmol/L, the molar absorption coefficient was 1.71 × 10^4 L·mol^-1· cm^-1, the recoveries ranged from 97.8% to 102.4%, The within- run and between- run CVs were 2.9% and 3.4%. Comparing this method （y） with MTB （x）, the regression equation y = 1.03x - 0.036 was obtained, r = 0.994 7, P 〉 0.05. The reference inteval （x^-±2 s） was 0.73 - 1.27 mmol/L （n = 51 ）. It concludes this method is simple, sensitive, rapid and accurate, and suitable for the routine manual or automatic analysis of magnesium in serum.
出处
《广东微量元素科学》
CAS
2006年第6期56-59,共4页
Trace Elements Science
基金
浙江省卫生厅青年基金资助课题(SQ950003)
关键词
血清镁
分光光度法
偶氮肿Ⅰ
serum magnesium
spectrophotometry
Arsenazo Ⅰ
