摘要
从NCBI公共数据库下载获得1989条茶树EST,去除其中的低质量的和冗余的序列,得到全长为734．54 kb的1589条无冗余EST。共在这些序列中发掘出了281个EST-SSR,分布于246条EST中,出现频率是17．68%。这些EST-SSR的平均长度为33．06 bp,平均分布频率是1/2．61 kb。茶树EST—SSR中,二核苷酸重复是主要的重复类型,出现最多的重复基元类型是AG/CT重复。本研究为茶树EST-SSR标记的建立和进一步应用奠定了基础。
1989 ESTs of tea plants were downloaded from the public database of NCBI and some of them redundant or with low quality were removed, finally resulting in 1589 non-redundant ESTs with total length of 734.54 kb. Totally 281 SSRs distributed in 246 ESTs were mined out, accounting for 17.68% of the non-redundant ESTs. The average length of tea plants EST-SSRs searched is 33.06 bp and the average distance of distribution is 1/2.16 kb. The dinucleotide repeat is the dominant type with repeat motif AG/CT being the most common. The result in this paper provides a base for the development and future application of EST-SSR markers in tea plants.
出处
《科技通报》
2006年第4期471-476,共6页
Bulletin of Science and Technology
基金
杭州市农业科学院资助项目(I20504)
