摘要
目的探讨甲醛对性成熟期雄性大鼠生殖的影响及其作用机制。方法选用性成熟期健康SD雄性大鼠40只,随机分为甲醛染毒高(10 m g/kg.d)、中(1 m g/kg.d)、低(0.1 m g/kg.d)剂量和对照组4组。腹腔注射染毒14 d后观察睾丸重量和形态学改变,精子数量和质量改变,血液中激素含量的改变;利用原位缺口末端标记法(TUNEL)检测睾丸细胞的凋亡;免疫组化方法检测睾丸F as基因的表达。结果①高剂量染毒组大鼠睾丸组织的质量明显低于对照组(P<0.05),中、高剂量染毒组睾丸曲细精管萎缩,生精上皮细胞层数减少,细胞排列紊乱;②中、高剂量染毒组大鼠精子的数量和精子活动度较对照组明显减少,精子畸形率明显增加(P<0.05);③染毒组与对照组相比,血清卵泡刺激素和黄体生成素含量轻度增高,而睾酮含量轻度下降,但差异没有统计学意义;④中、高剂量染毒组睾丸凋亡细胞较对照组明显增多(P<0.05),F as基因表达明显增加(P<0.05),且F as基因阳性表达与睾丸的凋亡指数呈正相关(r=0.8832,P<0.05)。结论甲醛对性成熟期雄性大鼠生殖系统有较明显的损伤,这种损伤可能与F as介导的睾丸生精细胞凋亡机制有关。
Objective To assess the reproductive toxicity exerted by formaldehyde to adult male rats and the functional mechanism therein in volved. Methods Forty adult male rats were randomLy divided into 4 groups. One group served as control. The other 3 groups as exposure groups were intraperitoneally injected with formaldehyde at the dose of 0.10 mg/kg·d, 1.00 mg/kg·d, 10 mg/kg·d respectively and continuously for 14 days. The weight and morphology of testis, the quality and amount of sperm, and the serum hormone were examined. In addition, the apoptosis rate of testis tissue was examined by TUNEL and the expression of Fas gene in testis tissue by immunohistochemistry. Results Compared with control, the weight of testis in the hlgh-dose formaldehyde exposure group significantly decreased （P 〈 0.05）; the convoluted tubules became atrophied, the layers of seminiferous epithelium decreased, the arrangement of seminiferous epithelium was in disorder in the medium- and high-dose formaldehyde exposure groups; the sperm count and sperm motility significantly decreased （P 〈 0.05）, the percent of abnormal sperm significantly increased （P 〈 0.05）in the medium- and high-dose formaldehyde exposure groups; the serum testosterone content mildly decreased and the serum FSH and LH content mildly increased in the formaldehyde exposure groups, but no statistically significant difference was observed. Moreover, in comparison with control, the apoptosis rate and the expression of Fas gene increased in the testicular tissue of rats in the medium- and high-dose formaldehyde exposure groups（P 〈 0.05）. The apoptosis rate was significantly correlated with the expression of Fas gene （r = 0.8832, P 〈 0.05）. Conclusion Formaldehyde exerts reproductive toxicity to adult male rats, and Fas-mediated apoptosis may be one of the functional mechanisms involved in the reproductive toxicity of formaldehyde.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第4期566-569,共4页
Journal of Sichuan University(Medical Sciences)
基金
陕西省科技计划重点项目(批准号2005K15-G2)
陕西省中医药管理局课题(批准号2005024)
西安交通大学自然科学基金(批准号573026)资助
关键词
甲醛
生殖
大鼠
凋亡
FAS
Formaldehyde Reproduction Rat Apoptosis Fas
