摘要
目的了解广州某医院秋冬季儿童腹泻中人类杯状病毒(HuCV)感染的分子流行病学特点。方法连续2个秋冬季腹泻流行季节收集临床诊断为病毒性腹泻患儿的粪便标本。采用ELISA检测轮状病毒,RT-PCR检测HuCV。部分阳性标本PCR产物进行纯化、测序,结合参考株相应核苷酸序列进行进化分析。结果诺瓦克样病毒(Norwalk-likevirus,NLVs)阳性率为12.35%(80/648),扎幌样病毒(Sapporo-likevirus,SLVs)阳性率为0.31%(2/648)。NLVs各月阳性率无显著性差异,主要感染2岁以下患儿。2003年流行株为GⅡ-3群和GⅡ-4群,2004年为GⅡ-4群。2株SLVs均为GⅠ-1群。结论NLVs是广州地区秋冬季节儿童腹泻的重要病原体,其流行株为GⅡ-3群、GⅡ-4群病毒株,但在不同的时间段可能存在不同的流行优势株;广州地区存在SLVs所致感染,与我国报告的SLV基因型不同。
Objective To investigate the epidemiological features of human calicivirus （HuCV） infection in children with diarrhea in a hospital in Guangzhou. Methods Stool specimens were collected from children with viral diarrhea diagnosed between October, 2003 and January, 2004 and between October, 2004 and January, 2005. HuCV was detected by means of RT-PCR and sequence analysis of the PCR products. Results Eighty specimens positive for Norwalk-like viruses （NLVs） were identified from 648 stool specimens, with a positivity rate of 12.35%, and sapporo-like viruses （SLVs） were identified in 2 specimens （0.31%）. The monthly NLV positivity rate was 11.74% （Oct.）, 14.16% （Nov.）, 9. 09% （Dec.） and 13.95% （Jan.）, respectively, showing no significant variation in these months. NLVs mainly infected children below 2 years old. Twenty-two strains of NLVs were sequenced and analyzed, and 21 of them were identified as GⅡ and the genotype of 1 strain could not be determined. The prevalent viral population were GⅡ-3 and GⅡ-4 in 2003 and was GⅡ-4 in 2004, and both of the SLVs belong to GⅠ-1. Conclusion NLVs is one of the important pathogens causing sporadic acute gastroenteritis in children admitted in the hospital in Guangzhou, and the prevalent strains axe GⅡ-3 and GⅡ-4, but different prevalent strains are possible in different periods.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第7期967-970,共4页
Journal of Southern Medical University
基金
广东省医学科研基金(A2003420)~~
