摘要
目的探讨甘遂对重症急性胰腺炎(SAP)大鼠胰腺组织微循环的影响及其机制。方法SD大鼠随机分为假手术组(S组)、SAP组和甘遂治疗组(K组),每组4 0只。检测各组手术后2,6,1 2,2 4 h的血清淀粉酶水平,胰腺组织TXB2和6-Keto-PGF1α含量、COX-2mRNA和蛋白表达水平、光镜和电镜观察胰腺组织结构,以及术后7 2 h死亡率。结果(1)胰腺组织TXB2,6-Keto-PGF1α水平及TXB2/PGF1α比值:SAP组在各时间点均较S组显著升高(P<0.0 1);K组TXB2及TXB2/PGF1α比值在6,1 2,2 4 h点均较SAP组显著降低(P<0.0 1),但仍高于S组(P<0.0 1)。(2)COX-2mRNA和蛋白表达:S组COX-2mRNA和COX-2蛋白表达均极弱;SAP组表达均明显;K组COX-2mRNA表达6,1 2 h明显弱于SAP组(P<0.0 5),COX-2蛋白表达6,1 2,2 4 h均显著低于SAP组(P<0.0 1)。(3)胰腺组织TXB2/PGF1α比值与COX-2蛋白表达呈显著正相关(r=0.8 6 7,P<0.0 1)。(4)光镜和电镜观察:S组胰腺组织结构正常;SAP组胰腺组织有出血坏死,微血管内大量血栓形成;K组胰腺组织损害较SAP组减轻,微血管内血栓明显减少。(5)7 2 h死亡率:S组为0%,K组为1 2.5%,两者均明显低于SAP组(6 2.5%)(均P<0.0 5)。结论重症急性胰腺炎时有COX-2的高表达和TXA2/PGI2之间的失衡,甘遂可以下调COX-2的表达,纠正TXA2/PGI2之间的失衡,而改善胰腺微循环。这可能是其治疗大鼠SAP的作用机理之一。
Objective To explore the effect and mechanism tissues in severe acute pancreatitis （SAP） rats. Methods of kansui root on microcirculation of pancreatic SD rats were randomly divided into sham group （ S group ） , SAP group and kansui root therapy group （ K group ） . 40 rats in each group. Serum amylase, and thromboxame-B2 （ TXB2 ） , 6-Keto-F1α （ 6-Keto PGF1α ） levels and expressions of cyclooxygenase-2 （COX-2） mRNA and protein in pancreatic tissue, microscopy and election microscopy of pancreas, mortality within 72 hour after operation in each group were tested at 2h, 6h, 12h, and 24h after operation. Results （1） The TXB2 ,6-keto PGF1α. levels and the ratio of TXB：/6-Keto-PGF1α （T/P） in SAP group were all obviously higher than those in S group （ P 〈 0.01 ） ; the TXB2 levels and the ratios of T/P in K group at 6 h, 12 h, and 24 h were significantly lower than those in SAP group （ P 〈 0.01 ） , but were significantly higher than that in S group （ P 〈 0.01 ）. （ 2 ） Expression of COX-2 mRNA and protein were rare in S group, but expression in SAP group was significant. In K group expression of COX-2 mRNA at 6h and 12h and expression of COX-2 protein at 6h, 12h, and 24h were significantly lower than those in SAP group （ P 〈 0.01 or P 〈 0.05 ）. （ 3 ） Correlation analysis ：the ratio of T/P was significantly correlated to COX-2 protein expression in pancreatic tissue （ P 〈 0.01 ）. （4） Microscopy and electron microscopy of pancreas： histologic structures of pancreas were normal in S group ; hemorrhage, necrosis and abundant thrombosis in microvessels were observed in pancreatic tissue in SAP group; in K group the pancreatic injury was mimer, and thrombosis in microvessels was decreased than that in SAP group. （5） Mortality within 72 hours in S group was 0 % , in K group was 12. 5 % , both were significantly lower than that in SAP group （ 62.5 % ） （ P 〈 0.05 ） . Conclusions There was high expression of COX-2 and imbalance between TXA2 and PGI2 in SAP rats. Kansui root may reduce the expression of COX-2 and by correction of the imbalance between TXA2 and PGI2 which can improve the microcirculation of pancreas. This may be one of the mechanisms of the effect of kansui root in treating SAP in rats.
出处
《中国普通外科杂志》
CAS
CSCD
2006年第6期432-437,共6页
China Journal of General Surgery
基金
湖南省中医药基金资助项目(204180)
关键词
胰腺/血液供给
微循环
胰腺炎
急性病
甘遂/药理学
Pancreace/blood supply
Microcirculation
Pancreatitis
Acute Diseases
Kansui/pharm
