摘要
目的观察大鼠不同孕期补碘所引起的高碘对仔鼠脑和智力发育的影响。方法受孕大鼠分成3个组,2个高碘组分别在受孕第0天和第7天一次性经口灌胃给予1 ml／kg 40％碘化油,对照组给予同等剂量食用豆油。观察仔鼠脑相对质量变化,脑切片HE染色测定锥体细胞密度;测量血清T3、T4水平、尿碘以及脑组织蛋白、一氧化氮(NO)水平等指标;用暗室电击实验测试学习记忆行为。结果①整个孕期对照组大鼠尿碘水平始终保持在800μg／L左右;高碘I组尿碘在灌胃后8 d始终高于40 000μg／L,随后逐渐下降,直到妊娠期结束时仍显著高于对照组;高碘Ⅱ组变化趋势基本同前。②各组仔鼠脑相对质量均持续减小,高碘I组第35天(PN35)与对照组比较差异有统计学意义(P<0．05),以PN21为界,在这之前高碘组仔鼠脑相对质量高于对照组,而后相反;海马锥体细胞密度变化同前。③2个高碘组仔鼠脑组织蛋白降低,NOS活性及NO水平增高,且避暗实验的潜伏期较短(P<0．05)。④高碘Ⅱ组仔鼠各指标与对照组比较差异无统计学意义(P>0．05)。结论孕期补碘性高碘对胚胎发育无不良影响,但可使出生后仔鼠的脑和智力发育受到抑制。
Objective To observe the effect of excess iodine administration during different time of pregnancy on brain development and intelligence of filial rats. Methods Pregnant rats were divided into three groups, 40% iodide oil was injected into stomachs of two excess iodine groups through mouth according to 1 ml/kg on pregnant day 0 and pregnant day 7 respectively. At the same time, edible bean oil was injected into control group in the same way. The change of relative brain weight was observed as well as their behaviour changes, using dark-room electric stroke test; the pyramidal cell density was measured with HE histochemistry, the T3,T4 of serum and nitric oxide （NO） content were determined. Results ①lodine content of urine in the control group remained the level of 800 μg/L all the time, in the first excess iodine group it declined gradually but was still higher than that in control group significantly at the end of pregnancy. The changing tendency of the second excess iodine group was similar to that of first group on the whole. ②Relative brain weight of each group was decreased gradually, there was a significant difference between the first group and control group at PN35 （P 〈 0.05）. Before PN21, relative brain weight and pyramidal cell density of the first group were higher than those of control group, but lower than those of control group after PN21. ③The brain tissue protein, brain NOS activation and NO content of the excess iodine groups were significantly different from those of the control group, and their latencies of escaping from dark were shorter（P 〈 0.05）. ④The second group was not significantly different from control group（P 〉 0.05）. Conclusions Excess iodine administration during pregnancy doesn＇ t affect rat embryos, but does affect filial rat brain development after birth.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2006年第4期389-392,共4页
Chinese Jouranl of Endemiology
基金
国家科技部"十五"科技计划项目(2001BA901A49)
关键词
妊娠
碘
脑
一氧化氮
Pregnancy
Iodine
Brain
Nitric oxide
