摘要
从种传番茄苗中获得一病毒分离物Ｔｏ－Ｓｌ，人工摩擦接种７科２４种植物，Ｔｏ～Ｓｌ能侵染４科１５种植物，在番茄上产生花叶，在白肋烟上为局部枯斑。Ｔｏ－Ｓｌ的钝化温度为８５～９０℃，稀释限点为１０￣（－６）～１０￣（－７）．体外保毒期在一个月以上。病毒粒体杆状，长度主要分布于２８１～３００ｎｍ之间，平均长度２８８ｎｍ。病毒衣壳蛋白亚基只有一条多肽链，分子量为２１ｋＤａ。ｄｓＲＮＡ分析测得其基因组长度为６．４ｋｂ。琼胎糖双扩散和胶内交叉吸附试验证明，Ｔｏ－Ｓｌ与ＴＭＶ有血清关系，但有一定的差异，病毒粒体电泳分析也表明Ｔｏ－Ｓｌ与ＴＭＶ粒体有差异。在交叉保护试验中，ＴＭＶ和Ｔｏ－Ｓｌ之间均无保护作用。根据以上试验结果Ｔｏ－Ｓｌ被鉴定为番茄花叶病毒。这是我国首次系统报道番茄上番茄花叶病毒的侵染。
virus isolate(To-Sl)from tomato was collected in Ha11gzhou,Zhejiang Province.To-Slcould infect 24 plant species of three families.To-Sl produced mosaic symptoms on tomato,andlocal leision on N.tabocum White Burley. Its thern1al inactivation point was 85～90℃,dilutionend point was 10￣(-6)～10￣(-7).The virus particles were rod-shaped with the average length of288nm.The molecular weight cf To-Sl coat protein estimated was 2 1 000 Da.dsRNA analysisshowed that To-Sl corLqisted of one single st rand RNA with the length of 6.4 kb.In double-diffusion tests,spur forn1ation was observed between To-Sl and TMV,and the resuIts of in-tragel cross-absorption tests agreed with the double-diffusion tests.In gel electrophoresis,thepatterns of To-Sl virions were different from TMV.To-Sl shared one bands with TMV,butTMV had another band with slow migration.Cross protect ion tests showed To-Sl had no prrvtection eHect to TMV,and vice versa.On the basis of these characteristics theTo-Sl was identi-fied as tomato mosaic virus(ToMV).This is the first report of ToMV on tomato in China.
出处
《中国病毒学》
CSCD
1996年第3期268-276,共9页
Virologica Sinica
