摘要
背景:内皮素、一氧化氮作为一对维持血管张力,血流动力的平衡因子,在不同的疾病过程中,均有不同的反应,阐明高脂血症对内皮细胞的影响及病理生理机制和寻找保护血管内皮的方法及药物已成为研究动脉粥样硬化的重点。目的:观察隔药饼灸对高脂血症兔血浆内皮素和一氧化氮含量的影响,分析隔药饼灸对高脂血症兔内皮素-一氧化氮的调节作用。设计:随机对照动物实验。单位:湖南中医药大学针推学院。材料:普通级健康新西兰白兔60只,体质量1.5～2.5kg,雌雄不拘。药饼由丹参、山楂、郁金、大黄、泽泻等按一定剂量比例碾为粉末,醋调为糊,制成直径5～8mm、厚2～3mm的药饼;艾炷:苏州东方艾绒厂,“神灸300灸”艾炷(型号:东方一号,批号:20021212)。方法:实验于2003-11/2004-10完成于湖南中医药大学实验动物中心。实验新西兰白兔随机分为4组,每组15只,即空白对照组、高脂血症模型组、直接灸组、隔药灸组。除空白组外以胆固醇高脂饮食喂养法,建立兔高脂血症模型。选两组穴位轮灸:Ⅰ组:巨阙、天枢(双)、丰隆(双)共五穴;Ⅱ组:心俞(双)、肝俞(双)、脾俞(双)共六穴。于实验开始第1天,选1组穴位,直接灸组兔将艾炷直接粘于穴位上点燃施灸,隔药灸组兔将去除底座的艾炷粘于置于穴位上的药饼上点燃施灸。每穴每次连灸4壮,每日1次,第二天换另一组穴位,两组交替,连灸40d。其余两组不予任何处理。于实验第41天,对各组动物进行血浆内皮素和一氧化氮含量的测定。采用放射免疫法测定治疗后内皮素的含量,硝酸还原酶法测定一氧化氮含量。主要观察指标:各组实验动物治疗40d后血浆内皮素和一氧化氮的含量。结果:实验过程中有5只兔因腹泻等原因死亡,其中空白对照组2只,模型组、直接灸组、隔药灸组各1只。最终进入结果分析55只。①隔药灸组血浆内皮素的含量明显低于模型组[(431.57±63.68),(500.14±75.41)ng/L,P<0.05],但隔药灸组与直接灸组内皮素含量差异无显著性意义[(431.57±63.68),(429.08±77.07)ng/L,P>0.05]。②4组血浆一氧化氮的含量虽然有模型组<空白组<隔药饼灸组<直接灸组的趋势,但4组之间两两比较经统计学处理差异无显著性意义[(27.17±16.55),(29.39±13.24),(30.24±20.25),(30.47±19.62)μmol/L,P>0.05]。结论:隔药饼灸和直接灸均对高脂血症兔血浆内皮素具有明显调节作用,且二者作用相近。对一氧化氮的调节作用不明显。
BACKGROUND： Endothelin and nitrogen monoxide （NO） which are regarded as a pair of factors to maintain equilibrium between vascular stress and hemodynamics have different responses during various diseases. It is the key point for the treatment of atherosclerosis to find out the effect and pathophysiological mechanism of hyperlipemia on endothelial cells, and seek the methods and drugs for protecting vascular endothelin.
OBJECTIVE： To observe the effect of herbal cake-separated moxibustion on contents of plasma endothelin and NO and analyze its regulative mechanism.
DESIGN： Randomized controlled animal study.
SETTING： Cgllege of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine.
MATERIALS： A total of 60 healthy New Zealand white rabbits weighing 115-2.5 kg of both genders were Selected in this study. Herbal cake was made of danshen, shanzha, yujin, dahuang and zexie, which were crushed into powder according to a certain ratio. Then, vinegar was added to make paste which was 5-8 mm in diameter and 2-3 mm in depth. Moxa cone： （Shenjiu 300） was provided by Suzhou Dongfang Airong Factory （type： Dongfang Ⅰ; batch number： 20021212）.
METHODS： The experiment was carried out in the Experimental Animal Center of Hunan University of Traditional Chinese Medicine between November 2003 and October 2004. All rabbits were randomly divided into 4 groups： blank control group, hyperlipemia model group, direct moxibustion group and herbal cake-separated moxibustion group with 15 in each group. Except blank control group, rabbits in other three groups were fed withhigh fat forage to establish hyperlipemia models. Two groups of aeupoints were acupunctured alternatively： Group Ⅰ： Juque （CV14）, Tianshu （ST25） at bilateral sides, and Fenglong （ST 40） at bilateral sides; Group Ⅱ： Xinshu（BL15）,Ganshu（BL18） and P/shu（BL20）, at bilateral sides respectively. On the first day, moxa cone was directly adherent to acupoints of rabbits in direct moxibustion group and lighten for acupuncture, but in herbal cakeseparated moxibustion group, moxa cone which was gotten rid of the carriage was adherent to herbal cake which was put on acupoints and lighted for acupuncture. Each acupoint was acupunctured for 4 successive dosages for once a day. On the next day, the other group of acupoints was acupunctured. The course was 40 days. Rabbits in other two groups were not treated with any ways. On the 41^th day, contents of plasma endothelin and NO were measured in each group with radio-immunity method and nitrate-reductase reduction method, respectively.
MAIN OUTCOME MEASURES： Contents of plasma endothelin and NO of rabbits after 40-day treatment.
RESULTS： Five rabbits died because of diarrhoea or other reasons, including 2 in blank control group, 1 in model group, 1 in direct moxibustion group and 1 in herbal cake-separated moxibustion group. Therefore, 55 rabbits were involved in the final analysis. ① Content of plasma endothelin was lower in herbal cake-separated moxibustion group than that in model group [（431.57±63.68）, （500.14±75.41） ng/L, P 〈 0.05], but there was no significant difference between herbal cake-separated moxibustion group and direct moxibustion group [（431.57±63.68）, （429.08±77.07） ng/L, P 〉 0.05]. ②On NO content, there was an increasing tendency of model group 〈 blank group 〈 herbal cake-separated moxibustion group 〈 direct moxibustion, but there was no significant difference between any two groups [（27.17±16.55）, （29.39±13.24）, （30.24±20.25）, （30.47±19.62） μmol/L, P 〉 0.05].
CONCLUSION： Effects of both two methods of moxibustion in the rabbits with hyperlipemia on content of endothelin are significant and similar, but there are no significant effects on NO content.
出处
《中国临床康复》
CSCD
北大核心
2006年第27期184-186,共3页
Chinese Journal of Clinical Rehabilitation
