摘要
本实验先制备大鼠肝再生模型，在该模型中大鼠成活率达９５％以上，肝再生情况良好，适合于进行下一步的研究。随后，通过耐热性和肝细胞特异性的检测，初步认为从该模型中所提取的活性成分即为肝再生刺激物（ＨＳＳ）。用３Ｈ胸腺嘧啶核苷测定ＨＳＳ及其ｍＲＮＡ体外翻译产物的生物活性，结果表明二者在肝再生过程中均存在动态变化，但前者在肝部分（２／３）切除后７２ｈ活性最高，后者则在２４ｈ达高峰。这一结果为后续的分子克隆工作奠定了基础。
A rat regenerating liver model was made, in which the survival rate was over 95%, the remained lobes regenerated normally after partial (2/3) hepatectomy (PH), and fit for the following study. By examining both the heat resistance and specificity of liver cells, it was reasonable to conclude that the active substance in the model was hepatic stimulatory substance (HSS). HSS both extracted from regenerating liver and translated from its mRNA was assayed for its biological activity by using 3 H thymidine. The level of both protein and mRNA of HSS was altered regularly after PH ,i.e., the former reached its peak at 72 hour after PH, but as to the later, the peak was at 24 hour after PH. These data provided experimental basis for the following molecular cloning study.
出处
《中国应用生理学杂志》
CAS
CSCD
1996年第3期236-238,共3页
Chinese Journal of Applied Physiology
关键词
肝再生刺激物
肝部分切除
肝再生模型
MRNA
hepatic stimulatory substance
partial hepatectomy
liver regenerating model
translating of mRNA in vitro
