摘要
采用３ＨＰＡＦ放射配体结合试验方法测定脊髓神经细胞膜上ＰＡＦ受体的特异性位点，观察脊髓损伤后２、６ｈ、１、３周脊髓神经细胞膜ＰＡＦ受体结合特性变化。结果显示，脊髓神经细胞膜上存在ＰＡＦ高、低亲和力结合位点，脊髓损伤后２、６ｈ、１周组ＰＡＦ受体高、低亲和力位点Ｋｄ值和Ｂｍａｘ均有不同程度下降，与对照组比较，有显著性差异（Ｐ＜０．０５）。表明在伤后早期ＰＡＦ受体亲和力增加，结合位点减少。提示ＰＡＦ受体在脊髓损伤后继发性损害病理生理过程中起一定作用。
The specific binding sites of platelet activating factor(PAF)on spinal cord neurocyte membrane of cats were measured by 3 H PAF radioligand binding test. The characteristic alteration of PAF receptor in spinal cord neurocyte membrane at 2, 6 hours, 1, 3 weeks after spinal cord injury were invesigated. The results revealed that there were both high affinity binding sites and low affinity binding sites of PAF in spinal cord neurocyte membrane. The Kd 1, B max1 , Kd 2, B max2 of PAF receptor on spinal cord neurocyte membrane at 2, 6 hours and 1 week after trauma significantly decreased in different degrees as compared to control group( P <0.05). indicating that the affinity of PAF receptor increased and binding sites decreased after trauma. It is suggested that PAF receptor playan important role in pathophysiological processes of sencondary damage after trauma.
出处
《中国应用生理学杂志》
CAS
CSCD
1996年第3期259-262,共4页
Chinese Journal of Applied Physiology
基金
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