摘要
采用传统分离手段对中国南海海域的细薄星芒海绵(Stellettatenui(Lindgren,1897))、皱皮软海绵(Halichondriarugosa(Ridley&Dendy))、澳大利亚厚皮海绵(Craniellaaustraliensis(Carter))、贪婪倔海绵(Dysideaavara(Schmidt))4种海绵体内的微生物进行了分离培养,随机挑选64株芽孢杆菌属细菌(每种海绵16株)进行了RAPD-PCR基因多态性分析。研究表明,一些海绵微生物是可以通过传统分离手段得到的,来自同一或者不同海绵的微生物均具有丰富的基因多态性。
sponge-associated microorganisms were isolated from 4 species of sponges Stelletta tenui （Lindgren, 1897） ,Halichondria rugosa （Ridley ＆ Dendy）,Craniella australiensis （Carter）,Dysidea avara （Schmidt）in the South China Sea. After cultivation the genetic diversity of 64 strains of sponge-associated Bacillus （16 strains/sponge） was revealed based on RAPD-PCR fingerprinting and clustering analysis. It was proved that we can obtain sponge-associated microorganisms by traditional isolation method and there is genetic diversity among strains from the same or different sponges.
出处
《海洋科学》
CAS
CSCD
北大核心
2006年第7期15-20,共6页
Marine Sciences
基金
国家863计划资助项目(2002AA628080
2004AA628060)
上海市科技启明星资助项目(04QMX1411)
