摘要
目的探讨用PCR-SSCP方法检测淋球菌氟喹诺酮耐药基因突变的研究。方法自行设计gyrA基因特异性扩增引物,运用PCR-SSCP银染色方法检测基因突变。结果在30例淋球菌临床分离株中选取13例,对其gyrA基因342 bp扩增产物进行DNA序列测定,其91位和95位位点发生突变。结论通过本研究所使用PCR-SSCP的方法检测临床分离出的淋球菌是否对氟喹诺酮耐药,并通过序列分析了解到淋球菌耐药基因突变以gyrA基因编码91位及95位点的氨基酸的碱基突变为主。通过此方法可以早期、准确、快速地检测出从临床获得的淋球菌菌株是否对氟喹诺酮耐药,从而指导临床用药及治疗。
Objective To evaluate the applicability of Polymerase chain reaction and Single-strand conformation polymorphism （PCR-SSCP）for detection of the Fluorquinolones resistance in Neisseris gonorrhoeae and mutation in the gene, Methods Design by oneself the gyrA gene primers specific,making use of the sliver dye PCR-SSCP examine the gene mutation. Results Among 30 clinical isolate of Ng elect to take 13 samples,and as to 342 bp of the gyrA gene products were purified and sequenced by DNA sequencer.Our firding showed that the mutations situated at codon 91 and codon 95. Conclusion Passing the PCR-SSCP method can examine Ng of clinical isolates whether frug resistance to the Fluoroquinilones. Passing the sequence analysis can show that the gene mutation of Ng with the gyrA gene coding of 91 with 95 nucleotide substitution.Passing this method can examine early.accurately.quickly Ng of clinical isolates whether drug resistance to the Fluoroquinolones,so that guide the clinical using the medicine and treatment.
出处
《中国实验诊断学》
2006年第7期697-699,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技厅科技发展项目资助课题(20010248)
