期刊文献+

5,7-二羟基-4'-甲氧基二氢黄酮与牛血清白蛋白的相互作用研究 被引量:16

Studies on the Interaction between 5,7-Dihydroxy-4'-methoxy- flavanone and Bovine Serum Albumin
下载PDF
导出
摘要 应用荧光光谱、紫外吸收光谱和核磁共振波谱研究了5,7-二羟基-4'-甲氧基二氢黄酮(ISO)与牛血清白蛋白(BSA)分子间的相互作用.研究表明:ISO对BSA内源性荧光的猝灭机制属于ISO和BSA形成化合物所引起的静态猝灭;二者的结合常数为7.41×1011L/mol,结合位点数为1.98.ISO与BSA作用的活性部位为其分子内的7-OH和5-OH,且7-OH活性强于5-OH,并且随着ISO浓度增大,BSA的构象发生了变化. The interaction between 5,7-dihydroxy-4′-methoxyflavanone (isosakuranetin, ISO) and bovine serum albumin isosakuranetin (BSA) has been investigated by fluorescence, UV absorption spectra and NMR spectroscopy. The results show that the mechanism of quenching the inner fluorescence of BSA is due to the combination of BSA with ISO which results in static quenching procedure. The binding constant is 7.41 ^- 10^11L/mol and the number of binding sites is 1.98. The active groups of ISO interacting with BSA are 7-OH and 5-OH, and 7-OH is more active than 5-OH. With the increase of ISO concentration, the conformation of BSA has been changed.
出处 《化学学报》 SCIE CAS CSCD 北大核心 2006年第13期1361-1366,共6页 Acta Chimica Sinica
基金 河南省杰出青年科学基金(No.0612001100)资助项目.
关键词 5 7-二羟基-4′-甲氧基二氢黄酮 牛血清白蛋白 相互作用 5,7-dihydroxy-4′-methoxyflavanone bovine serum albumin interaction
  • 相关文献

参考文献17

二级参考文献29

  • 1Gan, F.-Y. Chin. Pharm. J. 1992, 27(3), 138 (in Chinese).
  • 2Yang, C.-M. Chem. J. Chin. Univ. 2002, 23 (2), 243 (in Chinese).
  • 3Jiang, J.-G. ; Wang, Z.-X. ; Liu, C.-W. Chem. J. Chin.Univ. 2001, 22(7), 1131 (in Chinese).
  • 4Huang, B.; Zou, G.-L.; Yang, T.-M. Acta Chim. Sinica 2002, 60(10), 1867 (in Chinese).
  • 5Yang, M.-M.; Yang, P.; Zhang, L.-W. Chin. Sci. Bull.1994, 39(1), 31 (in Chinese).
  • 6Ma, G.-B.; Yang, P. Chin. J. Biochem. 1992, 8(5), 624(in Chinese).
  • 7Haris, P. I.; Severcan, F. J. Mol. Catal. B: Enzym. 1999,7,207.
  • 8Xie, M.-X.; Liu, Y. Chin. J. Anal. Lab. 2001, 20(Suppl.), 197 (in Chinese).
  • 9Purcell, M.; Neault, J. F.; Tajmir-Riahi, H. A. Biochim.Biophys. Acta 2000, 1478(1), 61.
  • 10Neault, J. F.; Novetta-Delen, A. ; Tajmir-Riahi, H. A. J.Biomol. Struct. Dyn. 1999, 17(1), 101.

共引文献210

同被引文献237

引证文献16

二级引证文献83

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部