摘要
目的:研究白细胞介素10(IL10)对血管升压素(AVP)诱导大鼠心脏成纤维细胞(CFs)增殖及p27蛋白表达的影响.方法:以培养的新生SD大鼠CFs为实验模型,四氮唑盐(MTT)比色法检测CFs增殖,流式细胞分析仪(FCM)技术测定细胞周期及p27蛋白表达阳性率.结果:①10-7mol/LAVP作用24h,CFs的A490为0.216±0.013,较空白组(0.132±0.006)明显增高(P<0.01).②10-11～10-8kg/LIL10+10-7mol/LAVP组A490分别为0.201±0.007,0.187±0.006,0.173±0.010,0.157±0.029,均明显低于10-7mol/LAVP组(其中10-11组P<0.05,10-10,10-9,10-8三组P均<0.01).③10-7mol/LAVP作用24h,CFs的S期百分率(12.3±0.7)及增殖指数(19.6±0.9)较空白组(4.2±0.5,7.1±0.6)均明显增高(P<0.01);10-9kg/LIL10+10-7mol/LAVP组S期百分率(9.6±1.1)及增殖指数(13.86±1.28)均明显低于AVP组(P<0.01).④10-7mol/LAVP作用24h,p27蛋白表达阳性率为(63.7±2.4)%,较空白组(78.5±2.5)%明显降低(P<0.01);10-9kg/LIL10+10-7mol/LAVP组p27蛋白表达阳性率为(70.5±2.4)%,较AVP组(63.7±2.4)%明显增高(P<0.01).结论:IL10具有抑制AVP诱导CFs增殖的作用,p27蛋白可能是IL10调控AVP诱导CFs增殖的细胞内靶蛋白.
AIM： To study the effect of interleukin-10（IL-10） on the proliferation and p27 expression in cultured cardiac fibroblasts （ CFs ） induced by arginine vasopressin （ AVP ）. METHODS ： The CFs of neonatal SD rats were isolated and cultured by trypsin digestion and selective plating technique. Cell number was evaluated by MTY assay （A490value）. Cell cycle distribution and p27 expression were determined with flow cytometry. RESULTS：① MTY colorimetry showed that 10^-7 mol/L AVP significantly increased A490 value of CFs（ 0. 216 ± 0. 013 ）in comparison with control group（0. 132±0.006, P〈0.01 ）. IL-10 can attenuate the A490 value of AVP group in a concentrationdependent manner. ② The A490 values of the 10^-11, 10^-10, 10^-9 ,10^-8 kg/L IL-10 ＋ 10^-7 mol/L AVP groups were 0. 201±0.007, 0. 187±0.006, 0. 173±0.010, 0. 157±0.029, respectively, all significantly lower than those of 10^-7 mol/L AVP group （10^-11kg/L IL-10 ＋ AVP group, P 〈 0.05; the others, P 〈 0.01）. ③The percentage of the cells in S stage（12. 3 ±0.7） and proliferation index （PI, 19. 6 ± 0. 9 ） were markedly increased in 10^-7 mol/L AVP group compared with those of the control （4.2±0.5,7.1 ±0.6, P〈0.01）. In the 10^-9 kg/L IL^-10 ＋ 10-7 mol/L AVP group, the percentage of the cells in S stage（9.6± 1.1 ）and PI（13.86± 1.28） were significantly lower than those of AVP group （ P 〈 0. 01 ）. ④ Positive rate of p27 expression in AVP group [ （ 63.7 ± 2.4 ） % ] were significantly lower than that of the control [ （78.5 ± 2.5 ）%, P 〈 0.01 ]. Positive rate of p27 expression of the 10^-9 kg/L IL-10 ＋ 10^-7 mol/L AVP group was （70.5 ± 2.41 ）%, significantly higher than that of AVP group [ （63.7 ±2.4）%, P 〈0.01 ]. CONCLUSION： IL-10 effectively inhibits the cell proliferation of CFs induced by AVP, and p27 may be the target regulatory protein of IL-10 in CFs.
出处
《第四军医大学学报》
北大核心
2006年第14期1256-1258,共3页
Journal of the Fourth Military Medical University
基金
陕西省自然科技基金资助项目(2004C221)
