摘要
目的研究丹皮酚对动脉粥样硬化家兔血管平滑肌细胞增殖的影响。方法高脂饮食法复制家兔动脉粥样硬化病理模型；HE染色并光镜观察主动脉病理学改变；MTT法分析体外培养的兔血管平滑肌细胞增殖水平；全自动生化分析仪测定主动脉组织中脂质含量；酶法测定SOD、MDA含量；放射免疫分析法测定IL-1B、TNF-α的含量；免疫组织化学法检测主动脉组织中PCNA的蛋白表达。结果丹皮酚（75、150mg·kg^-1）治疗给药（ig，6wk）能减轻模型动物主动脉内膜增厚及泡沫细胞数量；使主动脉组织中脂质、脂质过氧化反应以及炎性细胞因子含量等方面得到显著改善；并能显著抑制主动脉组织中PCNA蛋白的表达强度；丹皮酚（100—300mg·L^-1）可明显抑制体外培养的血管平滑肌细胞增殖反应。结论丹皮酚对血管平滑肌细胞增殖有明显抑制作用，可能是其降脂、减轻脂质过氧化反应以及炎性细胞因子含量等协同作用结果，并可能通过抑制PCNA的表达而调控动脉粥样硬化病变中的血管平滑肌细胞增殖周期。
Aim To study the effect of paeonol on proliferation of vascular smooth muscle cell in atherosclerosis rabbits. Methods Atherosclerosis model on rabbits was established by high fat diet. The pathologic changes of aortic blood vessel were observed by lightmicroscope. The effect of paeonol on the proliferation of vascular smooth muscle cell in vitro was analyzed by MTT method. The lipid, SOD and MDA contents in aorta tissue were assayed by employing the automatic biochemistry analyzer and enzymic method respectively. IL-1β, TNF-α were quantified by radio immunoassay. The expresstion of PCNA protein was assayed by immunohistochemical method. Results Treated with Paeonol （75, 150 mg· kg^-1） by interagastric administration for 6 weeks, the thickness of vascular tunica intima was observed and the number of foam cells was decreased in atherosclerosis rabbits. Paeonol（ 100 -300 mg · L^-1） could inhibit the proliferation of cultured vascular smooth muscle cell and diminish the level of TC, MDA, pro-inflammatory cytokines, as well as enhanc the concentration of SOD in aortic tissue of atherosclerosis rabbits. The expressing intensity of PCNA protein was weakened. Conclusion Paeonol could inhibit the proliferation of vascular smooth muscle cell in vivo and in vitro, which may be related to decrease of contents of lipid, the extent of lipid peroxidation and the concentrations of proinflammatory cytokines. Paeonol might regulate the proliferation periods of vascular smooth muscle cell through inhibiting the expression of PCNA protein.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第5期587-591,共5页
Chinese Pharmacological Bulletin
基金
安徽省优秀青年科技基金资助项目(No04043047)
安徽省自然科学基金资助项目(No0304002)
关键词
丹皮酚
动脉粥样硬化
血管平滑肌细胞
增殖
paeonol
atherosclerosis
vascular smooth muscle cell
proliferation
