摘要
目的:探讨基于人乳头瘤病毒抗原优势表位的治疗性多肽抗原组分、结构与诱导免疫应答之间的关系。方法:以芴甲氧羰基固相法合成多肽,用高效液相色谱分析多肽的纯度,对所合成的多肽采用质谱进行定性鉴定,用标准51Cr释放试验检测特异性细胞毒性T淋巴细胞活性。结果:合成的多肽经纯化后纯度都达到80%以上,经质谱分析,各肽的分子量测定值与理论值相符,所设计的治疗性多肽在体内外均诱导出较强的表位特异性细胞毒性T淋巴细胞活性。结论:在治疗性表位多肽设计中,将辅助性T细胞表位及脂类分子构建于分子内部可提高细胞毒性T淋巴细胞表位肽的免疫原性。
Objective：To explore the relation between these components and structure of the therapeutic peptide based human papillomavirus epitope and their triggering cytotoxic T cell. Methods ：These pepfides were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. The activity of specific CTL was measured by using a standard 4 h ^51Cr release assay. Results： All peptides were higher than 80% in purity and shown to have the expected molecular mass. The therapeutic lipopeptide could induce strong specific CTL activity in vitro and in vivo. Conclusion： The combination of CTL and T helper epitope and lipid molecule can remarkably improve the immunogenicity of CTL peptide.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第5期449-452,共4页
Chinese Journal of Immunology
关键词
子宫颈癌
表位
治疗性疫苗
Cervical cancer
Epitope
Therapeutic vaccine
