摘要
采用热恒温干燥法,研究不同脱水干燥温度对杉木种子质量的影响,通过超干燥处理后的种子贮藏1年、超低温保存、防冻剂预处理超低温保存不同含水量的杉木种子,分析其发芽率和种子活力,研究杉木种子贮藏后的质量变化.结果表明:50℃是杉木种子脱水的适宜温度,种子发芽率和活力指数最高;3%～5%的含水量是杉木种子超干燥保存的最适含水量,贮藏1年与常规低温贮藏效果没有显著差异.超低温保存表明,9%含水量的种子贮藏效果要比超干燥处理后贮藏好,可用于杉木种子的中长期保存.防冻剂超低温保存受防冻剂种类和浓度影响显著,尤其是二甲亚砜的浓度,冷冻方式和解冻方式的影响作用则被防冻剂削弱.
The influence of different desiccation temperature on the qualities of Cunninghamia lanceolata seeds was studied by thermal constant temperature desiccation method. The seed quality was tested by germination and vitality experiments for Cunningharnia lanceolata seeds after ultra - dried storage for 1 year and then treated by cryopreservation or by cryopreservation after being pretreated by cryoprotectants with different water contents. It was concluded that the rational desiccation temperature was 50℃, the treated seeds had the highest germination rate and vitality index. 3% -5% moisture contents of Cunninghamia lanceolata seeds were the most suitable for ultra - dried storage, and there was no significant difference in tested indexes within this scale of moisture contents after having been stored for 1 year by cryopreservation or by ordinary low - temperature storage. It was showed that the storage effects by cryopreservation was better than ultra - dried storage when the water content was 9%, which might be a good way for long term germplasm storage for Cunningharnia lanceolata. The types and concentrations of cryoprotectants, especially DMSO concentration, would significantly influence on the storage effect by cryopreservation. It was showed that the effects of freezing and thawing method were weakened by cryoprotectants.
出处
《西南林学院学报》
2005年第4期113-117,共5页
Journal of Southwest Forestry College
基金
国家自然科学基金资助项目(39670608)
教育部高等学校骨干教师计划基金资助项目(2000-65)
关键词
杉木
林木种子
超干燥贮藏
超低温贮藏
种子质量
Cunningharnia lanceolata
tree seeds
ultra - dried storage
cryopreservation
seed qualities
