摘要
高效液相色谱法同时测定水产品中色胺、2-苯乙胺、腐胺、尸胺、组胺、章鱼胺、5-羟色胺、酪胺、亚精胺和精胺。样品经高氯酸溶液提取，丹酰氯衍生化，C18柱色谱分离，以0．1mol／L醋酸铵和乙腈作流动相梯度淋洗，254nm紫外检测。方法检出限（SIN=3）：腐胺、亚精胺0．8μg／g；尸胺、组胺、酪胺、精胺1μg／g；章鱼胺、5-羟色胺2μg／g；2-苯乙胺3μg／g；色胺5μg／g。在0．2～10mg／L范围内，峰面积与质量浓度成良好的线性，相关系数r大于0．999。样品添加浓度在30、100和500μg／g的回收率为80％～111％，相对标准偏差为2．6％～15．8％。方法定量下限（LOQ）为30μg／g。本法简便、快速、灵敏、可靠。
A high-performance liquid chromatographic （HPLC） method was developed for the simultaneous determination of tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, octopamine, serotonin, tyramine, spermidine, and spermine in fishery products. The amines were extracted with perchloric acid solution and derivatized with dansyl chloride. The chromatographic separation was carried out on a C18 column（ 150 ×4. 6 mm）. A gradient elution program with a mixture of 0. 1 mol/L ammonium acetate and acetonitrile was used. The assay was validated by analyzing samples spiked with 30, 100 and 500 μg/g biogenic amines, respectively. The average recoveries and the relative standard deviations were in the range of 80% - 111% and 2.6% - 15.8% , respectively. The detection limits （ S/N = 3 ） were 0. 8 μg/g for putrescine and spermidine ; 1 μg/g for cadaverine, histamine, tyramine and spermine; 2μg/g for octopamine and serotonin; 3 μg/g for 2-phenylethylamine and 5 μg/g for tryptamine, respectively. This method is convenient, rapid, sensitive and reliable.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第4期59-62,共4页
Journal of Instrumental Analysis
基金
上海市科委项目(04DZ05018)
关键词
高效液相色谱
生物胺
水产品
High-performance liquid chromatography
Biogenic amines
Fishery products
