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RP-HPLC法测定妇炎洗液中盐酸小檗碱的含量 被引量:1

Determination of the Content of Berberine Hydrochloride in Fuyan Lotion With RP-HPLC
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摘要 目的:建立测定妇炎洗液中小檗碱含量的RP-HPLC方法。方法:以Kromasil KR100—5C18为分析柱,流动相为乙腈:0.1%磷酸(48:52,每100ml含0.1g十二烷基磺酸钠),检测波长365nm,样品用流动相稀释后进样分析。结果川、檗碱在0.2-2.2μg范围内线性关系良好(r=1.0000),平均加样回收率为98.21%,RSD为1.51%。结论:本方法能准确、快速测定妇炎洗液中小檗碱的含量。 Objective: To establish the method of RP - HPLC for determination of the berbefine hydrochloride in Fuyan lotion. Method: C18column was used with the mobile phase of acetonitrile : 0.1% orthophosphorie acid(48 : 52) at the detection wavelength of 365 rim. The sample was introduced and analyzed after diluting with mobile phase. Result: the berberine show a good linearity within 0.2 -2.2μg, r = 1. 0000, the average recovery was 98.21%, the RSD was 1.51%. Conclusion: the method can determine the content of berberine in the Fuyan lotion accurately and quickly.
出处 《中医药导报》 2006年第7期92-93,共2页 Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词 RP-HPLC 妇炎洗液 小檗碱 RP - HPLC Fuyan lotion Berberine
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