摘要
通过冷休克的方法诱导红鳍东方鲀的受精卵,孵化后的仔鱼,用PAS-Ⅲ型细胞流速仪测定其倍性,得到较高的三倍体诱导率。受精后8 min,0℃下处理15 min和受精后5 min,2℃下处理15min三倍体率最高,为100%;受精后8 min,4℃下处理10 min倍化率最低,为20%;其余几组的三倍体率为90%。正交试验结果表明,影响三倍体诱导率的主次顺序是处理时间、处理温度、处理起始时间。
The triploidy of Takifugu rupripes was induced by cold shock in this experiment. After the fertilized eggs were induced, they are incubated in sea water at a water temperature of 19 ℃ and hatched. The triploid rate was measured in DNA content in nuclei by flow cytometry. The maximum triploidy rate （ 100% ） was observed 8 minutes after fertilization, for 15 minute treatment at 0 ℃, and 5 minutes after fertilization, for 15 minute treatment at 2 ℃ ; while the minimum triploidy rate （20%） occurred 8 minutes after fertilization, for 10 minute treatment at 4 ℃. A triploidy rate of 90% was found in other treatments. The orthogonal design results showed that the triploidy rate was primarily affected by treatment duration, followed by treatment temperature and treatment duration
出处
《水产科学》
CAS
北大核心
2006年第7期349-352,共4页
Fisheries Science
关键词
红鳍东方纯
冷休克
三倍体
Takifugu rubripes
cold shock
triploidy
