摘要
利用RTPCR方法确认了Survivin基因在4周胎肝、胎脾、胎肾cDNA文库中,乳腺癌、胃癌、结肠癌组织中的表达,而在正常的肝组织中未见表达;利用分子克隆方法构建了重组表达质粒pBV220Survivin,并在DH5a菌株中成功表达了具有生物学活性的Survivin蛋白,体外检测到该重组蛋白与RhSmac具有良好的结合活性;经脂质体转染重组Survivin蛋白的L929细胞与对照相比具有更好的存活率。
Survivin expresssion in embryo spleen, embryo kidney, embryo liver and in many cancer tissues was determined by RT-PCR, while not in the health liver tissue. Construct the engineered Escherischia coli expressing human survivin and identify the expressed human survivin by Westen-blot. The combination activity of Survivin and RhSmac was determined in vitro. L929 cells transferred with Survivin can survive longer than which transferred with BSA.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2006年第5期54-57,共4页
China Biotechnology
