摘要
目的 获得黑斑蛤蚧和红斑蛤蚧两个类群6个线粒体12SrRNA基因片段序列，分析黑斑蛤蚧和红斑蛤蚧的进化关系，进而探讨蛤蚧种群的遗传变异，为蛤蚧的分类鉴定提供一定的科学依据。方法 测定蛤蚧Gekko gecko两个类群6个个体线粒体的12S rRNA基因片断序列。结合多疵壁虎G．jamnicus、无蹊壁虎G．swinhonis、铅山壁虎G．hokouensis同源DNA序列进行比较，进行分子系统学分析。结果 红斑蛤蚧阃变异范围为1．18％～1．91％；黑斑蛤蚧闻为2．36％～3．64％；红斑蛤蚧与黑斑蛤蚧之间为4．81％～6．73％；外群壁虎与蛤蚧内群问变异为30．44％～35．69％。结论 红斑蛤蚧与黑斑蛤蚧的差异是否达到了亚种级分化的水平，还有待进一步研宄。
Objective To obtain the sequences of mitochondrial 12S ribosomal RNA gene DNA fragment of 6 individuals from red Gekko gecko and black Gekko gecko of two different species which located in different places in order to explore the relationship and evolution of the black geckos and red geckos, and provide certainly scientific basis for gecko＇s classification.Mthods The mitochondrial 12S rRNA genes of 6 individuals of Gekko gecko were sequenced and compared with gckkonid species of G. japonicus, G. swinhonis, and G. hokouensis in terms of their DNA sequence similarity.Results The percentage of sequence divergence （sequence difference/sequence size） in the red tokay was 1.18% -1.91% ,in the black tokay was 2.36% -3.64% ,among the red tokay and the black tokay was 4.81% -6.73 %. Conclusion Further proof is needed to prove that the differences between red tokay and black tokay have reached subspecies grade level.
出处
《广西医学》
CAS
2006年第6期793-796,共4页
Guangxi Medical Journal
基金
广西壮族自治区科技厅科技攻关项目(桂科攻0235022-3)。
关键词
蛤蚧
12S
RRNA基因
进化关系
Gekko gecko
12S Mitochondrial rRNA gene, Phylogenetic analysis
