摘要
目的观察当归静脉注射液4种分离组分Angi-SY1、Angi-SY2、Angi-YS1和Angi-YS2对脑缺血模型大鼠的影响。方法取Sprague-Daw ley大鼠56只,随机分为模型组,假手术组,丹参注射液组,Angi-SY1、Angi-SY2、Angi-YS1和Angi-YS2组,每组8只。假手术组给予假手术,其他各组均采用结扎大鼠双侧颈总动脉的方法制作脑缺血模型。术后Angi-SY1、Angi-SY2、Angi-YS1和Angi-YS2组大鼠分别给予Angi-SY1、Angi-SY2、Angi-YS1和Angi-YS2注射液腹腔注射,12.48 mL.kg-1,qd;丹参注射液组大鼠给予丹参葡萄糖注射液腹腔注射,12.48 mL.kg-1,qd;假手术组大鼠和模型组大鼠给予等量0.9%氯化钠注射液腹腔注射,qd。2周后测定大鼠脑和血清中一氧化氮(NO)、脑组织超氧化物歧化酶(SOD)和血清丙二醛(MDA)的含量。结果与模型组比较,Angi-SY2、Angi-YS1、Angi-YS2组大鼠脑NO含量明显下降(均P<0.01),Angi-SY1组大鼠脑NO含量亦下降(P<0.05);Angi-SY1、Angi-SY2、Angi-YS1、Angi-YS2组大鼠脑SOD的含量显著升高(均P<0.01),其中Angi-YS2组升高最明显;Angi-SY2、Angi-YS2组大鼠血清中MDA含量均明显下降(均P<0.01),Angi-SY1组大鼠血清内MDA含量明显升高(P<0.01)。结论当归静脉注射液的分离组分Angi-SY2、Angi-YS1和Angi-YS2对大鼠缺血性脑损伤有治疗作用。
Objective To survey the effects of the 4 separate components （Angi-SY1, Angi-SY2, Angi-YS1 and Angi- YS1 ） of the Chinese Angelica root intravenous injection on the rat with a model of cerebral ischemia, Methods 56 SD rats were randomly divided into 7 equal groups： ① the model group, ②sham operation group, ③red sage root injection group, ④ Angi-SY, group, ⑤Angi-SY2 group, ⑥Angi-YS, group and ⑦ Angi-YS2 group. The model of cerebral ischemia was set up in -the rats by ligating bilateral common carotid arteries under urethan anesthesia except for the animals of the sham operation group that were subjected to the same operation but without ligation of the carotid arteries, after the operation, rats of groups④⑤⑥ and ⑦ were given each a daily intraperitoned injection of 12. 48 mL·kg^-1 of Angi-SY,, Angi-SY2, Angi-YS1 and Angi-YS2, respectively. Rats of group ③ were given each a daily intraperitoneal injection of 12. 48 mL·kg^-1 of red sage root glucose solution while animals of the model group （①） and sham operation group （②） received injections of equivalent amounts of 0.9% sodium chloride by the same route. 2 weeks later, the contents of nitric oxide in the brain and serum, the activity of superoxide dismutase （SOD） in the brain tissue and levels of serum melonyldialdehyde（MDA） were determined. Results In comparison with animals of the model group, rats of the groups Angi-SY2, Angi-YS, and Angi-YS2 had a much lower content of NO in the brain （ P〈0.01 ）. Rats of Angi-SY, group had also a lower NO content in the brain （ P〈0.05 ）. Rats of groups Angi- SYI, Angi-SY2, Angi-Ys, and Angi-YS2 had a significantly higher SOD activity in the brain tissue （P〈0.01 ）, and among these, rats of the Angi-YS2 showed the highest SOD activity. Animals of the groups Angi-SY2, Angi-YS2 showed a striking decrease in the serum MDA content （ P〈0.01 ） while rats of the Angi-SY, had a prominent increase in the content of serum MDA （ P 〈 0.01 ）. Conclusion The 3 components of the Chinese Angelica root intravenous injection Ang/-SY2 ,Angi-YS1 and Angi-YS2 were shown to exert protective effective effects on the rat with a model of cerebral ischemia.
出处
《医药导报》
CAS
2006年第8期729-732,共4页
Herald of Medicine
基金
湖北省科技攻关计划项目(基金编号:2001AA302B02)
武汉市技术创新与产业化重大专项攻关课题(基金编号:2002600513407)
