摘要
AIM： To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.METHODS： mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR- ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine. RESULTS： T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased. CONCLUSION： The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.
瞄准：在人的 T 星基因的 transfection 的 telomerase 活动调查效果进人的结肠癌房间的全身的感觉 cDNA 或部分反感觉 cDNA 衬里 HCT-116。方法：T 星基因的 mRNA 和蛋白质表示层次被 RT-PCR 和西方的污点决定，并且 telomerase 活动被 PCR-ELISA 测量，在 T 星的 transfection 以后感觉或反感觉基因进有 lipofectamine 的 HCT-116 房间。结果：T 星基因表示被提高或在 mRNA 和蛋白质两个都击倒层次，和 telomerase 活动显著地被增加或减少。结论：T 星基因可以以一种平行方式在人的结肠癌 HCT-116 房间参予 telomerase 活动的规定。
基金
Supported by the National Natural Science Foundation of China,No. 30271442, No. 39980010
