摘要
为了解肾综合征出血热(HFRS)时自然杀伤细胞(NK)活性及其相关因素的变化,采用MTT法、ELISA法和放射免疫分析法分别检测40例患者不同病期血中NK活性,β2-m和IL-2。结果显示,NK活性在发热期(52.81±10.89),低血压少尿期(58.02±13.70)比正常值(47.66±6.39)明显增高(分别为P<0.05,P<0.005);多尿期(43.34±7.70)和恢复期(50.23±9.46)逐渐下降并回复正常(均为P>0.05)。重危型患者NK活性在病初增高程度比轻中型患者更大(P<0.05)。血β2-m在发热期(6.84±2.36),低血压少尿期(8.20±3.04)和多尿期(4.93±2.72)均显著高于正常值(1.80±0.06)(P<0.05)。在少尿期,NK活性与β2-m呈直线正相关(P<0.05)。血IL-2(15.63±4.15)与正常值(14.5±6.99)相比无明显改变(P>0.05);与NK活性也无相关性(P>0.05)。这表明,HFRS时血NK活性和β2-m可能参与发病机制;它们的相关性暗示MHC-1类分子在激活NK中的可能作用。在该病中,未观察到IL-2与NK活性?
InordertostudythechangeofNKactivityanditsassociatedfactorsinpatientswithHFRS,weinvestigatedNKactivity,IL-2andβ2-mlevelinthebloodof40casesindifferentstagesusingmethodswithMTT,ELISAandradioimmunoassayrespectively.Incomparisonwithnormalvalue(47.66±6.39),theresultsshowedthatNKactivitiesincreasedremarkablyinfebrile(52.81±10.89),hypotensiveandoliguric(58.02±13.70)phases(P<0.05,P<0.005,respectively)whereasdecreasedgradualytonormalduringdiuretic(43.34±7.70)andconvalescent(50.23±9.46)phases(P>0.05respectively).Attheearlystageofthedisease,theincreasedegreeofNKactivitywasmuchhigherinseverecasesthanthatinmoderateormildcases(P<0.05).Thevalueofbloodβ2-mwasalsosignificantlyelevatedinfebrile(6.84±2.36),hypotensiveandoliguric(8.20±3.04)aswelasdiuretic(4.93±2.72)phases(P<0.05respectively).Therewasapositivelin-earcorrelationbetweenNKactivityandβ2-minoliguricphase(P<0.05).ThevalueofbloodIL-2(15.63±4.15)displayedneitherremarkablechange(P>0.05)norcorelationwithNKactivity.ThestudyindicatedthatthechangesinNKactivityandβ2-mmightparticipateinpathogenesisofHFRS.ThecorelationbetweenthemimpliedtheprobableroleofMHC-1moleculeintriggeringNKactivityinHFRS.ThestimulationofIL-2onNKfunctionwasnotobservedinthisdisease.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1996年第4期200-202,共3页
Chinese Journal of Infectious Diseases