摘要
目的探讨20(S)-原人参二醇(Ppd)对肝癌血管内皮生长因子(VEGF)及其基因表达的抑制作用。方法建立肝癌动物模型,将实验动物分为5组:对照组、环磷酰胺组、Ppd 25、50、100 mg/kg给药组,每组10只,给药2 w后处死动物,制成组织切片以备免疫组化及原位杂交。结果对照组肿瘤间质血管密度增高,VEGF及其mRNA呈高表达,且VEGF蛋白及mRNA的表达同肿瘤间质血管密度呈正相关,而Ppd给药组各指标均降低,且呈剂量依赖性,较对照组存在显著差异(P<0.01),且50 mg/kg以上剂量的抑制作用较环磷酰胺强(P<0.01)。结论Ppd能够抑制肿瘤组织中VEGF及其mRNA的表达,而抑制肿瘤血管的形成。
Objective To study the inhibitory effects of Protopanaxidiol （Ppd） on vascular endothelial growth factor （VEGF） and its mRNA expression of liver cancer. Methods Liver cancer model animals were divided into control group, positive drug group, cyclophosphamide group, low, medium and high dosage of Ppd group（25, 50, 100 mg/kg）, 10 ones in each group. After two weeks, the animals were killed to make histological section for immunohistochemical and hybridization in situ stain. Results Control group had increased tumor interstitial vessel density, the enhanced VEGF and mRNA expression positively relating to vessel density, while Ppd treatment group had decreased above indexes （P 〈 0.01 ） ; and medium and high dosage of Ppd had stronger inhibitory effect than cyclophosphamide （P 〈0. 01 ）. Conclusions Ppd inhibits VEGF and its mRNA expression in tumor tissue and tumor angiogenesis, thus inhibits tumor growth.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第7期945-946,共2页
Chinese Journal of Gerontology
基金
长春市科技局资助项目(2004072)
