摘要
运用紫外光谱法和荧光光谱法研究了有机染料麦塔喇红与牛血清白蛋白(以下简称BSA)的相互作用。紫外光谱法研究表明,两者之间的结合符合Scatchard模型,存在两类结合,结合数分别为8.4和11.1,结合常数分别为1.1×106mol-1.L和1.5×105mol-1.L。同时通过麦塔喇红与牛血清白蛋白的荧光猝灭对两者的作用机理进行了初步探讨,对蛋白质构象变化进行了分析。
In the present paper, the interaction of Magdala red and bovine serum albumin(BSA) was studied by UV-absorption and fluorescence spectrometry. The results of UV-absorption spectrometry show that the way of the interaction of Magdala red and BSA should obey the Scatchard Model. In other words, Magdala should be combined to some certain regions of BSA. Meanwhile, taking advantage of fluorescence quenching, the reaction mechanism of Magdala with BSA was explored. Protein molecule conformation change is discussed by synchronous fluorescence spectrometry.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第7期1314-1317,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金(30460117)
南昌大学校科研基金(z02478)资助项目
关键词
麦塔喇红
牛血清白蛋白
相互作用
光谱研究
Magdala red
Bovine serum albumin, Interaction
Spectrometry study