摘要
目的研究成鼠骨髓基质细胞体外培养的生长行为和分化情况。方法利用EGF、FGF-b等增殖及分化诱导因子和神经干细胞培养液进行培养,分化诱导,用细胞免疫组化染色进行细胞鉴定。结果成鼠骨髓基质细胞在体外培养中能形成细胞克隆团,具有增殖能力,并可分化出胶质细胞样细胞和神经元样细胞。结论骨髓基质细胞具有较强的自我更新及多向分化能力,在适宜的诱导分化条件下,可诱导为神经干细胞,分化出神经元和胶质细胞。
Objective To study the growth and differentiation of the cultured bone marrow stromal cells from adult rats. Methods BMSC from adult rats were cultured with EGF, FGF- b and neurabasal medium. Immunocytochemistry was performed to identify the differentiated cells. Results BMSC could expand and generate clones when they were cultured in vitro. These cells grew rapidly and differentiated into neuron-like cells and astrocyte-like cells. Conclusion BMSC can differentiate into neuron and astrocyte if the culture condition is appropriate.
出处
《中国实用神经疾病杂志》
2006年第4期54-55,共2页
Chinese Journal of Practical Nervous Diseases
关键词
骨髓基质细胞
神经干细胞
细胞培养
克隆
Bone marrow stromal cells
Neural stem cell
Cell culture
Clone
