摘要
血清白蛋白与萘啶酸的结合，可以猝灭萘啶酸－铽螯合体系的特征荧光，保泰松（Ｐｈｅｎｙｌｂｕｔａｚｏｎｅ）及布洛芬（Ｉｂｕｐｒｏｆｅｎ）等药物在白蛋白的特定结合位置与其具有较强的结合作用，因此，可用作白蛋白分子结合位置的标记物。本文以保泰松或布洛芬作标记物，探讨了牛血清白蛋白及人血清白蛋白对萘啶酸－铽螯合体系特征荧光的不同影响方式，并根据蛋白结合位上药物的置换作用确定了萘啶酸在牛血清白蛋白分子上的结合位置。
Through the binding with nalidixic acid,bovine serum albumin(BSA)and human serum albumin (HSA) could quench the characteristic fluorescence of nalidixic acid-terbium chelate. Because of the tight binding of phenylbutazone and ibuprofen to the special sites of albumins, they were used as the binding site markers on the albumins, In this paper the different effects of BSA and HSA on the fluorescence of nalidixic acid-terbium chelate were discussed. According to the displacement of the site markers on albumins, the binding site of nalidixic acid on the BSA has been found to be the Ⅱ special binding site.
出处
《高等学校化学学报》
CSCD
北大核心
1996年第12期1855-1858,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
