摘要
用高效液相色谱法紫外检测人尿中美托洛尔（Ｍ）和α－羟基美托洛尔（ＨＭ）浓度，采用ＳｐｈｅｒｉｓｏｒｂＯＤＳ１２５ｍｍ×４ｍｍｉ．ｄ．（５μｍ）色谱柱，甲醇：水：冰乙酸：三乙胺（７０：３０：０：１２：０：０３）为流动相，紫外检测波长为２１５ｎｍ。Ｍ和ＨＭ的平均回收率分别为９８．５／和９９．８％，日内和日间变异系数均小于４．５％。方法快速、简便、灵敏，可用于药物氧化分型研究。
Metoprolol (M) and α-hydroxymetoprolol (HM) in human urine were determined by using highperformance liquid chromatography with ultraviolet detection. Analytical column (125mm×4mm i. d.) was packed with Spherisorb ODS (dp= 5μm). The mobile phase consisted of methanol, water. glacial acetic acid and triethylamine (70 : 30 : 0.12 : 0.03, V/V). The ultraviolet absorption was monitored at 215nm. The mean recoveries of both compounds were 98. 5% and 99. 8%, respectively. The inter-day and intra-day variations of the results were within 4. 5%. This assay is well suited for phenotyping of debrisoquin-type drug oxidation in human populations.
出处
《色谱》
CAS
CSCD
北大核心
1996年第5期408-409,共2页
Chinese Journal of Chromatography
基金
NSFCC39200154
A39270794
F39330230和CMB92-568基金
关键词
高效液相色谱法
美托洛尔
药物氧化分型
high performance liquid chromatography, metoprolol, α-hydroxymetoprolol, human urine, phenotyping of drug oxidation
