摘要
用生物显微镜和扫描电子显微镜研究了红汁乳菇（Lactarius hatsutake Tanaka）子实体、担孢子、菌丝体的形态及与马尾松（Pinus massoniana Lamb.）形成的菌根的结构特征,并首次报道了红汁乳菇--马尾松形成的菌根菌丝套的网格结构.红汁乳菇担孢子大小为5.87～6.40×7.04～7.62 μm;表面棱脊高度0.30～0.45 μm,宽0.39 μm,相邻脊间最宽距离2.0 μm.菌丝套厚度平均为9.31 μm;哈蒂氏网平均厚度63.86 μm.菌丝直径、菌丝套厚度、哈蒂氏网厚度、菌根直径的比例为1∶4.7∶31.9∶138.4.菌丝套网格中网孔大多呈菱形,少数为不规则四边形.网孔长度为宽度的2.85倍,网孔宽度为网脊宽度的6倍.对菌根外延菌丝、斜面培养和液体培养菌丝的观测发现,红汁乳菇菌丝相邻细胞连接处没有锁状联合.
Morphological and structural characteristics of the fruit body, basidiospore and mycelium of artificially cultivated Lactarius hatsutake Tanaka and the ectomycorrhiza of L. hatsutakePinus massoniana were examined using light and scanning electron microscopy （SEM）. Basidiospores were 5.87～6.40×7.04～7.62 μm, and had surface ridges 0.30～0.45 μm high and 0.39 μm wide; the widest space between two adjacent ridges was 2.0 μm. The ectomycorrhiza surface was covered with a layer of light-colored and compact hyphae that formed a mycelial mantle with an average thickness of 9.31 μm, and the average thickness of the Hartig net was 63.86 μm. The mantle had a reticulate structure with holes that were mostly rhomboid and occasionally irregular quadrangular in shape. The length of the net hole was 2.85 times the width which, in turn, was six times the width of the net ridge. The ratio of the hyphal diameter, mycelial mantle thickness, Hartig net thickness and ectomycorrhizal diameter was 1∶4.7∶31.9∶138.4. No clamp connections were seen between adjacent hypha cells of the fungal mycelium irrespective of whether the hyphal emanated from the ectomycorrhiza, or from slant or submerged cultures grown on artificial media.
出处
《食用菌学报》
2006年第2期36-44,共9页
Acta Edulis Fungi
基金
科技部农业成果转化基金项目"松乳菇菌根苗规模化繁殖与栽培技术中试与示范"(03EFN216700295)的部分研究内容
关键词
担孢子
菌根
红汁乳菇
菌丝套
Basidiospore
Ectomycorrhiza
Lactarius hatsutake
Mycelial mantle
