摘要
胚胎干细胞(ESC)建系取材包括桑椹胚的卵裂球、囊胚的内细胞团(ICM)、上胚层细胞和原始生殖细胞(PGCs),甚至从新生鼠睾丸细胞也分离得到ES样细胞。传统观念常常把ESC等同于ICM细胞,也有学者认为ESC更像上胚层细胞,而在已知的基因标记方面,ESC所具有的特征更接近体内早期生殖细胞。不清楚ESC最接近的体内细胞类型与本质,可能是制约许多品系小鼠和大多哺乳类动物建系成功率提高的原因之一。综述了胚胎多能性细胞、早期生殖细胞和ESC的研究进展及相互关系,发现ESC可源于多种细胞类型。目前仍难以确定ESC最接近的体内细胞类型,进一步应通过研究不同体内细胞类型源ESC系间的差异,以弄清ESC本质,为提高ESC建系效率提供理论支持。
The inner cell mass （ICM）, blastomeres, epiblasts and primordial germ cells （PGCs） are usually used as primary materials for the establishment of embryonic stem cell （ESC） lines. ES-like cells have even been isolated from neonatal mouse testis. ESC are traditionally regarded as ICM cells, though some scholars believe they more closely resemble cells from the epiblast. However, recent evidence of ESC molecular markers indicate that the characteristics of ESC resemble those of early germ cells. The unknown origin and nature of ESC may limit the successful establishment of ESC lines from many different species. Here we review the progress of research regarding embryonic pluripo- tent cells, early germ cells and ESC. We find ESC can be derived from many cell types. Future study should elucidate the origin of ESC by comparing different ESC lines so as to determine the nature of ESC and improve the efficiency of ESC derivation.
出处
《遗传》
CAS
CSCD
北大核心
2006年第8期1037-1042,共6页
Hereditas（Beijing)
基金
科技部"863"项目(编号:2002AA216161)
国家自然科学基金项目(编号:30200137)资助~~
