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流动注射阻抑化学发光法测定己烯雌酚 被引量:3

Flow injection chemiluminescence method for the determination of diethylstilbestrol with N-bromosuccinimide-luminol system
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摘要 基于在碱性介质中,己烯雌酚对N-溴代丁二酰亚胺-鲁米诺化学发光体系的阻抑作用,建立了测定己烯雌酚的流动注射化学发光分析新方法,探讨并优化了流动注射化学发光的分析条件。该方法测定己烯雌酚的线性范围为1.5×10^-8~2.6×10^-7g/mL,检出限为5.0×10^-9g/mL,对1.0×10^-7g/mL的己烯雌酚标准溶液进行11次测定,相对标准偏差为2.6%。并应用于饲料、己烯雌酚片剂中的己烯雌酚的测定。 In alkaline medium, a novel flow injection chemiluminescent method has been developed for the determination of diethylstilbestrol, based on the inhibition of diethylstilbestrol on the chemiluminescence reaction between luminol and N-bromosuccinimide. Conditions of this chemiluminescent reaction have been discussed and optimized. The linear range of determination is 1.5×10^-8~ 2.6×10^-7 g/mL for diethylstilbestrol, the detection limit is 5.0×10^-9 g/mL, and the relative standard deviation (RSD) (n = 11 ) is 2.6% for 1.0× 10^-7 g/mL diethylstilbestrol. The method has been applied to determine the content of diethylstilbestrol in feed and medicine with satisfactory results.
出处 《分析试验室》 CAS CSCD 北大核心 2006年第8期10-12,共3页 Chinese Journal of Analysis Laboratory
基金 国家自然科学基金资助(20277005)
关键词 N-溴代丁二酰亚胺 鲁米诺 己烯雌酚 化学发光 流动注射分析 N-Bromosuccinimide Luminol Chemiluminescence Diethylstilbestrol Flow injection analysis
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