摘要
目的:探讨氧化苦参碱(OMT)对小鼠变态反应性接触性皮炎(ACD)的抑制作用。方法:56只BALB/c小鼠随机分成7组,每组8只。除B组(该组小鼠不致敏,也不诱发ACD)外,其他6组均建立ACD模型。A组每d腹腔注射20ml/kg的PBS,B、C、D、E、F、G组分别每d腹腔注射OMT50mg/kg、12.5mg/kg、25mg/kg、50mg/kg和100mg/kg及氢化可的松25mg/kg,均给药28d。于给药第8d、14d、29d测各组小鼠左、右耳厚度,计算2耳差值。实验第1d、7d、14d、21d、28d小鼠尾静脉采血三色免疫荧光标记,以流式细胞仪检测各组CD4+CD25+T细胞数。第29d处死全部小鼠,测量胸腺及脾脏质量。结果:OMT具有强烈抑制DNFB诱发的小鼠耳部ACD作用,且OMT可增加各剂量组小鼠外周血中CD4+CD25+T细胞数,呈剂量依赖性。小剂量的OMT可以使小鼠胸腺和脾脏质量增加,而大剂量的OMT对小鼠脾脏质量的影响不明显,但能降低小鼠胸腺的质量。结论:OMT显著升高小鼠外周血中CD4+CD25+T细胞数,可能是OMT抑制ACD的免疫学机制之一。
Aim： To explore the inhibitive effects of oxymatrine（OMT） on allergic contact dermatitis（ACD） in mice. Methods：A total of 56 BALB/c mice were allocated into 7 groups, 8 mice in each group. Except for group B, group A, C, D, E, F, and G were established ACD model, respectively. Group A was intraperitoneally injected PBS at 20 ml/kg per day, group B, C, D, E, and F were intraperitoneally injected OMT at 50 mg/kg, 12.5 mg/kg, 25 mg/kg, 50 mg/kg and 100 mg/kg, respectively per day,and group G was given hydrocortisone at 25 mg/kg. All the groups were given medicine for 28 days. The ear swell-degree of mice was measured on the 8th day, 14th day, and 29th day and the differential swelling rate of the two ears was calculated;peripheral blood of mice were collected via tail-vein on the 1st day,7th day, 14th day, 21st day, and 28th day, and CD4^＋CD25^ ＋T cells were counted with flow cytometer. On the 29th day, all mice were killed and the fresh-weight of thymus and spleen were measured. Results： Compared with group A, OMT could inhibit the ACD induced by DNFB and increase the percentage of CD4 ^＋ CD25 ^＋ T cells in a dose-dependent way. Low-dose OMT could increase the weight of thymus and spleen of mice,and high-dose OMT could decrease the weight of thymus. Conclusion ： OMT can improve the number of CD4 ^＋ CD25 ^＋T cells in the peripheral blood of mice, which may be a main immunological mechanism of OMT restraining ACD in mice.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2006年第4期643-645,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30571684
