摘要
目的观察普罗布考对动脉粥样硬化斑块形成及可溶性血栓调节蛋白的影响。方法将新西兰大白兔给予高胆固醇饮食8周后随机分为淀粉组、普罗布考组(给予普罗布考每天500 mg/kg)和对照组。第14周末处死动物进行主动脉病理学检测,酶联免疫吸附法测定可溶性血栓调节蛋白浓度。结果高胆固醇饮食8周后,总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇水平较0周时明显升高,普罗布考干预6周后胆固醇水平显著小于淀粉组(P<0.05),较8周时明显降低(P<0.01)。高胆固醇饮食14周后淀粉组主动脉可见明显的斑块形成,普罗布考组斑块面积虽较对照组增厚,但显著小于淀粉组(51.54%±8.32%比84.81%±9.35%,P<0.01);淀粉组主动脉内膜较对照组显著增厚,普罗布考组主动脉内膜厚度虽较对照组厚,但显著小于淀粉组(0.58±0.17 mm比1.62±0.18 mm,P<0.01)。正常饮食兔的可溶性血栓调节蛋白水平很低(0.65±0.25μg/L),高胆固醇饮食后逐渐升高,与淀粉组比较普罗布考干预6周后可溶性血栓调节蛋白水平显著降低(4.31±1.45μg/L比9.82±1.55μg/L,P<0.01);血浆可溶性血栓调节蛋白水平与动脉粥样硬化斑块的面积呈正相关(r=0.72,P=0.008)。结论普罗布考延缓动脉粥样硬化斑块的形成作用可能与其降脂及降低可溶性血栓调节蛋白水平,保护内皮功能有关。
Aim To explore the effects of probucol on the plaques formation of aorta and soluble thrombomodulin in hypercholesterolemic rabbits. Methods 16 male New Zealand white rabbits were fed with 1% cholesterol, 7.5% protein, 8% lard diet for 8 weeks, and then were randomly divided into two groups. Starch group （ n = 8 ） ： maintained cholesterol and starch （500 mg/kg everyday） diet for 6 weeks; probucol group （ n = 8） ： the same cholesterol diet supplemented with （500 mg/kg everyday） probucol for 6 weeks; andcontrolgroup（n=8） was fed with normal diet for 14 weeks. The soluble thrombomodulin concentrations were detected by enzyme-link-immuno sorbent assay, and the histopathological changes of the aortas were detected at the end of the study. Results Compared with control group, rabbits fed with high cholesterol diet showed higher levels of serum total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol, all of which were significantly reduced by probucol treatment. There were atherosclerotic lesions in aortas in starch group as compared with control group. The areas of the plaque （51.54%±8.32% ） and the intima thickness （0.58 ±0.17 mm） of the aorta were ameliorated in probucol group as compared with starch group （84.81% ± 9.35 %, 1.62 ± 0.18 mm, respectively ）. The base concentrations of soluble thrombomodulin in the three groups were very low. The levels of sTM were significantly increased after 10 weeks of high-cholesterol diet in starch group, and probucol significantly reduced the level of soluble thrombomodulin （3.25 ± 0.52 μg/L） as compared with starch group （7.56±0.62μg/L） at the end of the study. The concentration of soluble thrombomodulin was posifively asso- ciated with the plaques areas of aorta （ r = 0. 72 , P=0.008）. Conclusions Probucol decreased the plasma cholesterol and soluble thromomodulin concentrations and protected the endothelial function, which may inhibit the formation of plaques of aortas in cholesterolemic rabbits.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第6期503-507,共5页
Chinese Journal of Arteriosclerosis
