摘要
目的观察静脉注射内毒素所致大鼠急性肺损伤(ALI)模型的病理生理学指标,全面评价该模型。方法将33只Wistar大鼠随机分为实验组和对照组,对照组于静脉注射生理盐水后2h处死动物,实验组静脉注射革兰阴性菌脂多糖后分别于2、4和6h后处死,比较各组死亡率、呼吸频率、肺脏病理、血气指标、肺顺应性、右肺湿重/体重比值、血清和支气管肺泡灌洗液(BALF)中肿瘤坏死因子α(TNFα)水平。结果实验组6h动脉血氧分压(PaO2)跌至69.18mm Hg(1mm Hg=0.133kPa);肉眼肺脏可见明显淤血、出血点和水肿;光镜下肺泡正常结构消失,间质水肿增宽,大量炎性细胞浸润,毛细血管明显扩张、充血,白细胞附壁;肺顺应性跌至正常值的47%,右肺湿重/体重比值相当于正常值的137%;血清、BALF中TNFα水平急剧升高。结论以肺部特征性病理改变和PaO2下降大于30%(与基线值相比)作为判定大鼠ALI模型是否成功的主要指标;以肺顺应性、湿重/体重比值作为辅助指标,可能更适合于大鼠ALI模型。
Objective To observe and evaluate the pathophysiological indexes of acute lung injury （ALI） induced by lipopolysaccharide （LPS） in rats. Methods Thirty-three Wistar rats were randomly divided into normal control group and experiment group. Respiratory rate , mortality, arterial blood gases, compliance and wet weight of right lung/body weight ratio, tumor necrosis factor -α （TNF -α） in serum and bronchoalveolar lavage fluid （BALF） were determined 2, 4 and 6 hours after injection of LPS or normal saline in both groups. Results In the experiment group,the following changes were found. Arterial partial pressure of oxygen （PaO2） was reduced to 69.18 mm Hg （1 mm Hg= 0. 133 kPa）, marked blood stasis, and edema in lung tissues could be grossly seen and pathological examination showed that there was a large number of inflammation cell infiltration and edema in interstitial spaces with disappearance of normal construction of alveolae. There was also dilatation of capillaries with congestion and adherent leukocytes. Furthermore, compliance was decreased to 470% of the normal value, and wet weight of right lung/body weight ratio increased to 137% of the normal value. Blood TNF -α level increased markedly in serum and BALF. Conclusion Specific pathological changes and decreased PaO2 over 30% of the baseline value are the main signs of successful reproduction of ALI model in rats. Compliance and weight of right lung/body weight value can also reflect the status of ALI as helpful indexes.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第8期485-487,F0007,共4页
Chinese Critical Care Medicine
基金
国家"十五"重大攻关课题(2003BA712A0701)
关键词
内毒素
肺损伤
急性
模型
lipopolysaccharide
acute lung injury
model
