摘要
目的优选金银花中总黄酮乙醇提取最佳工艺。方法建立金银花总黄酮含量测定的比色法,并利用正交试验设计,以总黄酮得率为指标,考察乙醇浓度、乙醇用量、提取时间和提取次数对金银花总黄酮乙醇提取工艺的影响。结果最佳提取工艺为金银花粉末用12倍量90%的乙醇回流提取3次,每次1h。结论该提取工艺条件简单、稳定、可行。
Objective To study of the process for extracting total flavone of Lonicera Japonica Thunb. Methods A colorimetric method to determine total flavone of Lonicera Japonica Thunb was established. Orthogonal design was used for studying the effect of alcohol concentration, volume, refluxing times and duration. Results The optimum extracting technical conditions were as follows： 12 times of 90% alcohol, refluxing 3 times and one hour each time. Conclusion This optimized process is simple, stable and efficient.
出处
《安徽医科大学学报》
CAS
北大核心
2006年第4期410-412,共3页
Acta Universitatis Medicinalis Anhui
基金
安徽医科大学校科研基金资助项目(编号:200404)
关键词
金银花/化学
正交试验/方法
提取法
flos lonicerae/chemistry
orthogonal test/methods
extraction
