摘要
目的观察循环内皮祖细胞(EPC)数量和急性脑卒中及脑血管危险因素的关系。方法以CD133和KDR双阳性细胞作为EPC标记,对74例急性脑卒中患者(脑卒中组)和29例健康志愿者(正常对照组)的外周血淋巴细胞进行流式细胞分析,检测EPC数量;并检测血脂及高敏C反应蛋白(hs-CRP)。结果调整年龄、性别、收缩压及血清低密度脂蛋白胆固醇(LDL-C)后,与正常对照组比较,脑卒中组EPC数量明显减少,hs-CRP水平显著升高(P<0.01);EPC数量与年龄、收缩压、舒张压、血清总胆固醇、甘油三酯、LDL-C、hs-CRP均呈负相关(P<0.001),与性别及高密度脂蛋白胆固醇水平无关(P>0.36);多因素逐步回归分析显示,收缩压和LDL-C与EPC数量独立相关。结论循环EPC数量减少与急性脑卒中显著相关,收缩压与LDL-C水平是影响循环EPC数量的主要独立因素。
Objective To investigate whether the number of the endothelial progenitor cells （EPC） might be associated with stroke and cerebrovascular risk factors. Methods EPC were identified as the CD133/KDR double positive cells by flow cytometry analysis. The number of circulating EPC was analyzed in 74 patients with acute stroke and in 29 healthy subjects. Serum lipids and high-sensitive CRP （hs-CRP） were measured with automated biochemical machine. Results After adjustment for age, sex, systolic blood pressure and serum low-density lipoprotein cholesterol, stroke patients had a significant lower number of EPC and a higher level of hs-CRP compared with healthy subjects （ P 〈 0.01 ）. The number of circulating EPC was inversely （P 〈 0.001） correlated with age, systolic and diastolic pressures, serum total cholesterol, triglyceride, LDL-C, and hs-CRP, but not with sex and high-density lipoprotein cholesterol （ P 〉 O. 36）. Multiple stepwise regression analysis showed that systolic blood pressure and LDL-C independently correlated with the EPC number, respectively. Conclusion The diminished circulating EPC was associated with acute stroke. Systolic blood pressure and LDL-C are significant and independent factors that may influence the number of circulating EPC.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2006年第8期510-512,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
关键词
脑血管意外
内皮
血管
C反应蛋白质
脂蛋白类
LDL
危险因素
cerebrovascular disorders
endothelium, vascular
C-reactive protein
lipoproteins, LDL
risk factors
