摘要
目的探讨还原型谷胱甘肽对大鼠肺缺血再灌注后肺细胞凋亡的影响。方法雄性清洁级大鼠72只,随机分为3组,假手术组(shamoperation,SO)(n=24)、缺血再灌注组(ischemia-reperfusion,IR)(n=24)、还原型谷胱甘肽治疗组(reducedglutathionegroup,RG)(n=24),每组随机分为4个亚组(n=6)。参照Okada的方法,建立原位阻断肺门的大鼠肺缺血再灌注损伤模型。RG组在缺血前5min注射还原型谷胱甘肽800mg/kg,IR组与SO组注射等量的生理盐水。缺血45min、再灌注后1,2及4h4个时点处死大鼠进行指标测定。凋亡细胞的检测使用TUNEL技术;Bcl-2蛋白表达采用SP法测定;采用ELISA法测定10%肺匀浆中TNF-α含量。结果大鼠肺缺血再灌注损伤后,IR组肺组织细胞凋亡明显高于SO组和RG组(均P<0.01),再灌注后RG组Bcl-2蛋白表达明显高于SO组和IR组,TNF-α含量在IR组缺血再灌注后含量明显增加,较SO,RG组显著增高(P<0.01)。结论还原型谷胱甘肽能减少肺缺血再灌注后肺细胞的凋亡。
Objective To observe the effects of reduced glutathione on apoptosis induced by pulmonary isehemia-reperfusion in rats . Methods Seventy-two male SD rats were randomly divided into 3 groups： the sham groups（SO, n=24）; theischemia-repeffusion group（IR, n = 24） ;the reduced glutathione-treated group（RG, n = 24）,and each group was randmnly subdivided into 4 subgroup（ n = 6）. IR injury was in duced by in situ pulmonary hilum occlusion. The group RG was treated by reduced glutathione （800 mg/kg） 5 rains before ischemia, while other two groups were treated by normal saline.Pulmonary tissues were obtained 45 rains after ischemia and 1, 2, 4 h after repeffusion. Apoptosis was investigated by TUNEL; The expression of Bcl-2 were measured by inununobistochemical technique（SP）;ELISA was used to determine the level of tumor necrosis factor-alpha （TNF-α）.Results In IR group,pulmonary apoptosis was significantly higher increased than that in the SO and RG group（ P 〈 0.01）after ischemia-repefusion. In RG group, expression of Bcl-2 was significantly higher than that in the S and IR group （ P 〈 0.01） after ischemia- repeffusion. TNF - α in IR group was significantly higher than that in the SO and RG group. Conclusion Reduced glutathione can decrease pulmonary apoptosis after ischemia-repeffusion.
出处
《广东医学》
CAS
CSCD
北大核心
2006年第8期1133-1135,共3页
Guangdong Medical Journal
基金
贵州省科技计划项目(编号:黔科合专字[2003]33-3)
